基于LC-MS技术的婴幼儿脾虚型迁延性腹泻尿液代谢谱研究

Study on urine metabolic spectrum of children with infantile persistent diarrhea due to spleen deficiency based on LC-MS

目的:研究婴幼儿迁延性腹泻的尿液代谢谱变化及其发病机制。方法:本研究分为两组,正常对照组30例,脾虚泻组30例,以3个月到3岁婴幼儿尿液为样本,鉴定并分析尿液样本的内源性代谢产物,基于液相色谱-质谱联用技术(LC-MS)的代谢组学方法,通过主成分分析法(PCA)及正交偏最小二乘法-判别分析(OPLS-DA)进行内源性物质分析。结果:最终筛选并鉴定出L-天冬酰胺、苏氨酸、L-色氨酸、L-谷氨酰胺、L-赖氨酸、L-组氨酸、琥珀酸等63个内源性差异性代谢物,涉及苯丙氨酸代谢,丙氨酸、天冬氨酸和谷氨酸代谢,组氨酸,精氨酸生物合成的紊乱。结论:迁延性腹泻患儿与正常婴幼儿尿液代谢存在差异,迁延性腹泻过程涉及氨基酸代谢紊乱,为进一步阐释本病的发病机制提供了科学依据。

Objective: To study the urinary metabolic spectrum changes and pathogenesis of children with infantile persistent diarrhea due to spleen deficiency. Methods: This study was divided into two groups, 30 cases of normal children group and 30 cases of persistent diarrhea due to spleen deficiency group. The urine metabolic spectrum of children with persistent diarrhea from 3 months to 3 years old was studied. The endogenous metabolites of urine samples were identified and analyzed.Based on liquid chromatography-mass spectrometry(LC-MS), analysis of endogenous substances were performed by principal component analysis(PCA) and orthogonal partial least squares discriminant analysis(OPLS-DA). Results: A total of 63 endogenous metabolites were screened and identified, including L-aspartate, threonine, L-tryptophan, L-glutamine, L-lysine,L-histidine, succinic acid, and so on. It involved the disorder pathways of phenylalanine metabolism, alanine, aspartate and glutamic acid metabolism, histidine, and arginine biosynthesis. Conclusion: There are differences in urine metabolism between children with persistent diarrhea and normal children, and the process of persistent diarrhea involves amino acid metabolism disorder, which provided scientific basis for further elucidating the pathogenesis of the disease.