siRNA抑制牛睾丸细胞Nrf2基因表达的研究

Inhibition of Nrf2 Gene Expression in Primary Bovine Testicular Cells by siRNA

为了探讨Nrf2基因被干扰后对牛睾丸细胞的影响,试验采用了实时荧光定量PCR和Western boltting方法检测了牛睾丸细胞中HO-1和NQO1基因的表达。结果显示,设计的siRNA-1672在终浓度为50nmol/L时抑制效果较好,Nrf2基因表达显著下降,HO-1和NQO1基因在mRNA水平上表达显著下降。Nrf2被干扰72h后,HO-1和NQO1蛋白质水平表达水平明显下降。说明Nrf2对牛睾丸细胞中HO-1和NQO1基因的表达存在调控作用。

In order to investigate the effect of Nrf2 gene interference on primary bovine testicular cells, the expression of HO-1 and NQO1 in primary bovine testicular cells were detected by real-time quantitative PCR and Western bolting. The results showed that the designed siRNA-1672 had a better inhibitory effect at the final concentration of 50 nmol/L, the expression of Nrf2 gene decreased significantly, and the expression of HO-1 and NQO1 genes was significantly decreased at the mRNA level. After 72 hours of interference, the expression levels of HO-1 and NQO1 protein decreased significantly. This indicates that Nrf2 regulates the expression of HO-1 and NQO1 genes in primary bovine testicular cells.