人血浆与大鼠同/异源性血浆诱导TRALI大鼠模型比对研究

A Comparative Study of TRALI Rat Model Induced by Human Plasma and Homo/Heterologous Rat Plasma

目的比较研究人血浆和大鼠同/异源性血浆诱导建立TRALI大鼠模型可行性与有效性。方法SD大鼠腹腔内注射脂多糖后,分别静脉输注SD大鼠、BN大鼠及人血浆,采集临床症状、肺组织湿干重比、肺组织病理学数据。结果输注人血浆组大鼠均出现明显的TRALI相关临床症状;输注BN血浆组大鼠中只有60%出现不同程度的TRALI相关临床症状;输注SD血浆组大鼠几乎均未出现TRALI相关临床症状(P<0.05)。另外,输注人血浆组大鼠肺组织湿/干重比及肺损伤病理评分与输注SD血浆组大鼠差异有统计学意义(P<0.05);输注BN血浆组与SD血浆组大鼠肺损伤病理评分差异也有统计学意义(P<0.05)。结论输注人血浆能有效诱导建立TRALI SD大鼠模型,且具有重复性和稳定性。

Objective To compare the feasibility and validity of TRALI rat models induced by human plasma and rat homo/heterologous plasma respectively.Method After intraperitoneal injection of lipopolysaccharide in SD rats,SD rats,BN rats and human plasma were intravenously infused,and clinical symptoms,wet-dry ratio of lung tissue,and lung histopathological data were collected.Result All rats in the human plasma infusion group showed obvious TRALI-related clinical symptoms.Only 60%of the rats in the BN plasma infusion group showed different degrees of TRALI-related clinical symptoms.Almost none of the rats in the SD plasma infusion group showed TRALI-related clinical symptoms(P<0.05).In addition,the wet-dry ratio of lung tissue and the pathological scores of lung injury in SD plasma group were significantly different from those in human plasma group(P<0.05).There was significant difference in pathological scores of lung injury between BN plasma group and SD plasma group(P<0.05).Conclusion Infusion of human plasma can effectively induce TRALI-SD-rat model with repeatability and stability.