营养基质对植物乳杆菌转化纳米硒的影响

Effect of Nutrient Substrate on the Nano-selenium Translated by Lactobacillus plantarum

植物乳杆菌在生长繁殖中可转化合成低毒、高效的纳米硒,常见的营养基质对植物乳杆菌转化合成纳米硒具有较大的干扰。试验通过改良培养基、洗涤前处理避免基质对纳米硒转化的影响。结果表明:培养基中葡萄糖、乳糖具有还原性,可将亚硒酸钠转化合成纳米硒。改良MRS培养基适合菌体生长,且利于纳米硒的合成,当亚硒酸钠质量浓度为4 g/L,富硒率较高,合成的纳米硒颗粒均匀、稳定、分散性好。采用植物乳杆菌菌体洗涤后接种于磷酸缓冲液中可促进亚硒酸钠转化合成纳米硒,胞内可见纳米颗粒,既可避免基质干扰,也为研究植物乳杆菌转化纳米硒奠定基础。

Lactobacillus plantarum can synthesize nano selenium with low toxicity and high efficiency during its growth and reproduction.Common nutrient substrates have great interference on the transformation of Lactobacillus plantarum into nano selenium.The experiment aims to avoid the influence of substrate on nano selenium transformation by modified medium and pre-washing treatment.The results showed that glucose and lactose have reducibility,which can convert sodium selenite into nano-selenium;the modified MRS medium was suitable for the growth of Lactobacillus plantarum and also conducive to the synthesis of nano selenium.When the mass concentration of sodium selenite was 4 g/L,the selenium enrichment rate was higher.The synthesized nano selenium particles were uniform,stable and well dispersed;Lactobacillus plantarum washed and inoculated in phosphate buffer can promote the conversion of sodium selenite into nano-selenium,and nanoparticles can be seen in the cell,which can avoid matrix interference and lay the foundation for the research on the transformation of nano-selenium by Lactobacillus plantarum.