ε-聚赖氨酸盐酸盐对贮藏期间双孢蘑菇表面微生物生长和保鲜的影响

Effects ofε-Polylysine Hydrochloride on Microorganisms Growth and Freshness of Agaricus bisporus Fruiting Bodies during Storage

以延长双孢蘑菇货架期为目标,考察不同温度(4,16,25℃)条件下,不同质量浓度(0.15和0.30 g/L)的ε-聚赖氨酸盐酸盐(ε-PL)对采后双孢蘑菇表面背景菌群及假单胞菌生长的影响,建立货架期预测模型。贮藏期间,分析感官评分、白度评价、硬度评价、失重率及水分含量等指标的变化,研究ε-PL对双孢蘑菇的保鲜性能。结果表明:4,16和25℃时,双孢蘑菇在不同质量浓度的ε-PL(0.15和0.30 g/L)作用下,背景菌群及假单胞菌的生长曲线分为3个阶段(迟缓期、对数期和稳定期)。在4℃条件下,ε-PL对双孢蘑菇表面假单胞菌生长有抑制作用,质量浓度为0.30 g/L的ε-PL对双孢蘑菇表面假单胞菌生长抑制作用最佳,对双孢蘑菇的保鲜性能最优。

This research was designed to investigate the shelf life of Agaricus bisporus fruiting bodies during storage.The effect of different concentrations ofε-Polylysine hydrochloride(0.15 and 0.30 g/L;ε-PL)on the growth of background microorganisms and Pseudomonas spp.on Agaricus bisporus fruiting bodies were established at 4℃,16℃and 25℃,respectively.And the shelf life prediction model of Agaricus bisporus fruiting bodies was built.In the meantime,the samples of Agaricus bisporus fruiting bodies were collected during the storage to investigate the score of sensory,L*value,hardness,weight loss rate,and moisture content.Results showed that treatment withε-PL(0.15 and 0.30 g/L)could interfere with bacterial growth on Agaricus bisporus fruiting bodies.The background microorganisms and Pseudomonas spp.on Agaricus bisporus fruiting bodies were modeled with three different phases:lag phase,log phase,and stationary phase.Theε-PL could inhibit the growth of Pseudomonas spp.on the surface of Agaricus bisporus fruiting bodies at 4℃.Theε-PL(0.30 g/L)showed the strongest inhibitory against Pseudomonas spp.on the surface of Agaricus bisporus fruiting bodies compared to the other concentration ofε-PL(0.00 and 0.15 g/L).The study suggested that the combination ofε-PL and low temperature could effectively improve the quality and the shelf life of Agaricus bisporus fruiting bodies during storage.