大孔树脂纯化黑果腺肋花楸多酚的工艺研究

Purification of polyphenols from Aronia melanocarpa by macroporous adsorption resin

以黑果腺肋花楸为原料,采用大孔树脂纯化黑果腺肋花楸中的多酚类物质。从AB-8、ADS-17、HPD-100、NKA-Ⅱ4种大孔树脂中筛选出对黑果腺肋花楸多酚吸附-解吸效果最好的一种树脂,通过单因素试验确定大孔吸附树脂纯化黑果腺肋花楸多酚的静态吸附-解吸最佳工艺条件。结果表明:AB-8大孔吸附树脂对多酚的吸附-解吸效果最好,其静态吸附-解吸最佳工艺条件为:吸附时间3 h、上样液pH=4、解吸时乙醇体积分数70%;动态吸附-解吸最佳工艺条件为:上样流速2.0mL/min、上样量80mL、洗脱流速1.0 mL/min、洗脱体积60mL。在该工艺条件下,黑果腺肋花楸多酚纯度由10.80%提高到52.20%,表明AB-8大孔吸附树脂对于纯化黑果腺肋花楸多酚有较好的效果。

The polyphenols in Aronia melanocarpa were purified by macroporous resin.From AB-8、 ADS-17、 HPD-100 and NKA-II,the best resin was selected for adsorption and desorption of polyphenols from Aronia melanocarpa.The optimum static adsorption desorption conditions of polyphenols from Aronia melanocarpa were determined by single factor experiment.The results showed that AB-8 macroporous resin had the best adsorption desorption effect on polyphenols.The optimal static adsorption desorption conditions were as follows:adsorption time was three hours,pH was four,and ethanol volume fraction was70%;dynamic adsorption desorption process conditions were as follows:sample loading flow rate was 2.0 mL/min,sample volume was 80 mL,elution flow rate was 1.0 mL/min,and elution volume was 60 mL.Under the above conditions,the purity of polyphenols from sorbus nigricans was increased from 10.80% to 52.20%,which indicated that AB-8 macroporous resin had a good effect on the purification of polyphenols from Aronia melanocarpa.