右旋糖酐酶的吸附和固定方法及其对生物膜的影响

Adsorption and Immobilization Methods of Dextranase and Effects on Biofilm

为较好地消除生物膜,采用BCA法和DNS法分别探索羟基磷灰石吸附和固定化右旋糖酐酶的最适条件及酶活,以吸附和固定化右旋糖酐酶的羟基磷灰石干预变异链球菌生物膜.结果显示:最佳吸附比为1∶20,吸附时间为1 h,吸附率可高达23.9%;最佳的固定化条件为0.02%戊二醛交联2 h,固定化率最高可达17.6%,最高酶活分别为0.32 U/mg和0.35 U/mg.在上述条件下,吸附和固定化右旋糖酐酶的羟基磷灰石均能较好地清除变异链球菌生物膜的完整性.

In order to eliminate biofilm better, the adsorption of hydroxyapatite and immobilization of dextranase were studied respectively with the methods of BCA and DNS to get the optional condition and enzyme activities, then the hydroxyapatite adsorbed or immobilized by dextranase was used to interfere the biofilm of Streptococcus mutans. The results showed that the best adsorption ratio of hydroxyapatite to dextranase was 1∶20;the adsorption time was 1 h, and the adsorption rate reached 23.9%. The best immobilization condition was 0.02% glutaraldehyde cross-linked for 2 h, and the immobilization rate reached 17.6%. The highest enzyme activity reached 0.32 U/mg and 0.35 U/mg, respectively. Under the above conditions, hydroxyapatite that adsorbed or immobilized by dextranase can better eliminate the integrity of biofilm of Streptococcus mutans.