生物荧光酶抑制法快速定性测定粮食中有机磷类和氨基甲酸酯类农药残留

Rapid Qualitative Determination of Organophosphorus and N-methylcarbamate Pesticide Residues in Grain Based on Bioluminescence Inhibition of Enzyme

建立了生物荧光酶抑制法测定粮食及其制品中的有机磷类及氨基甲酸酯类的农药残留快速定性检测方法。样品通过乙酸乙酯∶丙酮(体积比为95∶5)提取离心净化,取50μL上清液45℃氮气吹干,1 mL蒸馏水或去离子水复溶进行检测。结果表明,本方法测定粮食及其制品中的有机磷类及氨基甲酸酯类农药残留时,最低检测限为0.1μg/kg,提取液与酶溶液的最佳反应时间是10 min,提取液与酶溶液的最佳反应温度为35℃。通过确立不同农药的限量抑制率,可以提高判定的准确度。本方法同时处理7个样品需要20 min,检测时间为15 min,平均每个样品5 min即可出结果,可用于粮食及其制品中有机磷类及氨基甲酸酯类农药残留的快速筛选。

A rapid qualitative method for the determination of organophosphorus and N-methylcarbamate pesticide residues in grain and its products by bioluminescence inhibition of enzyme was developed.The sample was extracted by ethyl acetate:acetone(volume ratio=95∶5)and centrifuged.The 50μL supernatant was dried with nitrogen at 45℃.The solution was redissolved with 1 mL distilled water to be tested.The method LOD was 0.1μg/kg,the optimum reaction time was 10 minutes,and the optimum reaction temperature was 35℃.The determination accuracy could be improved by establishing the limited inhibition rate of different pesticides.This method could be used for the rapid screening of organophosphorus and N-methylcarbamate pesticide residues in large quantities of grain and its products.It took 20 minutes to process 7 samples simultaneously,and the detection time was 15 minutes.The results could be obtained within 5 minute on average for each sample.