牛血清白蛋白/壳聚糖双层修饰载肉桂醛脂质体的制备

Preparation of cinnamaldehyde loaded liposomes bilayer-modified by bovine serum albumin/chitosan

目的 制备牛血清白蛋白(BSA)/壳聚糖(CTS)双层修饰的载肉桂醛(CA)脂质体(BSA/CTS-Lip-CA),以提高脂质体纳米粒子对药物的缓释效果和储存稳定性。方法 采用薄膜分散法制备载药脂质体(Lip-CA)和空白脂质体(Lip-Blank),然后利用静电吸附作用制备CTS修饰脂质体(CTS-Lip-CA)和BSA/CTS-Lip-CA。对所制脂质体进行表征,并考察其体外释药特性和储存稳定性。结果 所制BSA/CTS-Lip-CA的粒径为(177.8±4.0)nm、Zeta电位为(-15.6±1.5)mV,呈类球形;傅里叶红外光谱分析结果显示,BSA、CTS的修饰未对脂质体的内部结构产生影响。体外释药特性考察结果显示,Lip-CA、CTS-Lip-CA、BSA/CTS-Lip-CA在10 h内的累积释放量分别为82.9%、74.1%、72.9%。储存稳定性考察结果显示,储存30 d后,Lip-CA、CTS-Lip-CA和BSA/CTS-Lip-CA的粒径分别为(134.2±2.1)、(151.7±0.4)、(164.8±1.5)nm,其对模型药物CA的保留率分别为65.4%、82.5%、90.2%。结论 成功制得BSA/CTS-Lip-CA;其具有一定的缓释作用,且可在一定程度上提高药物的储存稳定性。

OBJECTIVE To prepare cinnamaldehyde(CA) loaded liposomes bilayer-modified by bovine serum albumin(BSA)/chitosan(CTS)(BSA/CTS-Lip-CA) in order to improve the sustained-release effect and storage stability of the nanoparticles. METHODS Firstly,cinnamaldehyde loaded liposomes(Lip-CA) and blank liposomes(Lip-Blank) were prepared by thin film dispersion method. Then chitosan modified cinnamaldehyde loaded liposome(CTS-Lip-CA) and BSA/CTS-Lip-CA were obtained by electrostatic adsorption. Finally, the prepared liposomes were characterized, and their in vitro release characteristics and storage stability were investigated. RESULTS The particle size of BSA/CTS-Lip-CA was(177.8±4.0)nm and the Zeta potential was(-15.6±1.5)m V;they were in spherical shape;FTIR analysis showed that the modification of BSA and CTS had no effect on the internal structure of liposomes. The results of in vitro drug release characteristics showed that the cumulative release of Lip-CA,CTS-Lip-CA and BSA/CTS-Lip-CA within 10 hours were 82.9%,74.1% and 72.9% respectively.The results of storage stability showed that after 30 days of storage,the particle sizes of Lip-CA,CTS-Lip-CA and BSA/CTS-Lip-CA were(134.2±2.1),(151.7±0.4),(164.8±1.5)nm;the retention rates of model drug CA were 65.4%,82.5% and90.2% respectively. CONCLUSIONS BSA/CTS-Lip-CA is successfully prepared. It has a certain sustained-release effect and can improve the storage stability of the drug to a certain extent.