两种不同结香时间的栽培奇楠沉香化学成分研究

Study on the Chemical Constituents of Cultivated"Qi-Nan"Agarwood Harvested at Two Different Formation Times

为了解不同结香时间对奇楠沉香品质的影响,利用气相色谱—质谱联用方法(GC-MS)分析了结香时间分别为12个和18个月的奇楠沉香样品的乙醚提取物成分及其相对含量。采用冷浸超声法用乙醚提取奇楠沉香样品,两份样品的浸膏得率分别为32.40%(12个月)和37.20%(18个月)。利用GC-MS分析其化学成分,并通过峰面积归一化法确定各化学成分的相对含量再进行对比,共检测出20种不同的化学成分。其中,结香时间为12个月的样品中共检测出19种化学成分,占总相对含量的97.29%;结香时间为18个月的样品中共检测出14种化学成分,占总相对含量的99.53%。其化学成分结构类型主要有2-(2-苯乙基)色酮类化合物和倍半萜类化合物,且以2-(2-苯乙基)色酮类化合物为主,其相对含量分别为86.66%(12个月)和92.22%(18个月);倍半萜类成分虽然相对含量较低,分别为10.07%(12个月)和7.31%(18个月),但结构类型较丰富,发现有桉烷型、艾里莫芬烷型、愈创木烷型、沉香螺旋烷型和β-檀香烷型。

In order to investigate the effects of different formation times on the quality of"Qi-Nan"agarwood,two ether extracts of agarwood samples with formation time of 12 and 18 months were collected,and then they were analyzed by gas chromatography-mass spectrometry(GC-MS).The cold immersion ultrasonic method was used to extract two agarwood samples with ether,and the extract yields of the two samples were 32.40%(12-month one)and37.20%(18-month one),respectively.The chemical constituents were analyzed by GC-MS,and their relative contents were determined by peak area normalization method and then compared.Totally,20 different chemical constituents were identified.Among them,a total of 19 chemical constituents were detected from the sample with the formation time of 12-month one accounting for 97.29%of the total relative contents;while a total of 14 chemical constituents were detected from the sample with the formation time of 18-month one,accounting for 99.53%of the total relative contents.The main structural types of chemical constituents were 2-(2-phenylethyl)chromones and sesquiterpenes,and 2-(2-phenylethyl)chromones were dominant in relative contents.Its relative contents were 86.66%(12-month one)and 92.22%(18-month one),respectively.Although the relative contents of sesquiterpenoids were relatively low,with 10.07%(12-month one)and 7.31%(18-month one),they presented structural diversity,consisting of eudesmane,eremophilane,guaiane,agarospirane,andβ-santalane.