持续水分胁迫对丹参茎叶酚酸含量及其抗氧化活性的影响

Effect of Continuous Water Stress on Phenolic Acids Content and Antioxidant Activity in Stems and Leaves of Salvia miltiorrhiza Bge.

丹参种植中产生大量废弃茎叶,造成巨大的资源浪费,干旱显著影响药用植物活性成分的产生和积累。该研究以‘川丹参1号’为材料,采用土壤水分胁迫法,探讨长期水分胁迫对丹参茎叶的总酚酸(TPA)、8种主要酚酸含量和乙醇提取物抗氧化活性的影响,以初步明确丹参茎叶中酚酸及其抗氧化能力对土壤水分胁迫的响应特征,为丹参茎叶的开发利用提供理论依据。结果表明:(1)丹参茎叶TPA对土壤水分响应敏感,8、9、10月份样品(SL-8、SL-9和SL^(-1)0)的TPA含量随着土壤相对含水量(SRWC)降低而增加,且不同月份间差异显著;在相同SRWC下,SL-9的TPA含量最低,仅为SL-8的41.4%~91.1%和SL^(-1)0的24.0%~79.9%。(2)长期土壤水分胁迫下,丹参茎叶中迷迭香酸、丹酚酸B、咖啡酸和原儿茶醛明显积累;干旱增加了TPA和单一酚酸的含量,但严重缺水(SRWC为35%)会降低酚酸含量。(3)丹参茎叶乙醇提取物具有很强的抗氧化活性,其DPPH·和ABTS·+清除活性随着SRWC的降低而增强,与TPA含量变化趋势一致。研究发现,适度干旱胁迫能显著增加丹参茎叶酚酸含量及抗氧化活性,在丹参种植中可以通过科学灌溉技术来增加茎叶中酚酸含量,促进丹参的综合利用。

The planting scale of Salvia miltiorrhiza continues to expand,producing many stems and leaves,resulting in a huge waste of resources.Drought significantly affects the production and accumulation of active components in medicinal plants.This study used‘Chuan Danshen 1’(CDS-1)as the material and used the soil water stress method to study the effects of long-term water stress on the total phenolic acids(TPA),the contents of 8 major phenolic acids and the antioxidant activity of the stem and leaf ethanol extracts.This study preliminarily clarified the response behavior and antioxidant capacity of phenolic acids in S.miltiorrhiza stems and leaves to soil water stress,and provided scientific theoretical guidance for the development and utilization of S.miltiorrhiza stems and leaves.The results showed that:(1)TPA of CDS-1 stems and leaves was sensitive to soil moisture.The TPA content of August,September and October samples(SL-8,SL-9 and SL^(-1)0)increased as the soil relative water content(SRWC)decreased,but there are significant differences between different months.Under the same SRWC,SL-9 has the lowest TPA content,only 41.4%-91.1%of SL-8 and 24.0%-79.9%of SL^(-1)0.(2)Under long-term soil water stress,the rosmarinic acid,salvianolic acid B,caffeic acid and protocatechuic aldehyde were significantly accumulated in the stems and leaves of CDS-1.Drought stress increases the contents of TPA and single phenolic acid,but severe water shortage(SRWC is 35%)will reduce the content of phenolic acid.(3)Ethanol extracts of the CDS-1 stems and leaves had strong antioxidant activity,and their DPPH·and ABTS·+scavenging activities increased with the decrease of SRWC,which was consistent with the change trend of TPA content.It was found that moderate drought stress significantly increased the phenolic acid content and antioxidant activity of the CDS-1 stems and leaves.Scientific irrigation technology can be used to increase the phenolic acid content of the stems and leaves in the cultivation of S.miltiorrhiza to promote the comprehensive utilization of S.miltiorrhiza.