‘阳光玫瑰’雄性不育株花蕾发育期抗氧化酶活性和内源激素含量的变化

Analysis of Antioxidant Enzymes Activities and Endogenous Hormone Contents in Male Sterile Plant of‘Shine Muscat’Seeding during Bud Development

以葡萄品种‘阳光玫瑰’及其实生后代雄性不育株‘Y-14’为试材,研究花蕾发育期抗氧化酶活性和内源激素含量的变化,阐明葡萄雄性不育新种质的生理生化特征。结果表明:(1)与‘阳光玫瑰’相比,雄性不育株‘Y-14’花蕾的超氧化物歧化酶(SOD)、过氧化物酶(POD)、过氧化氢酶(CAT)活性显著降低,而丙二醛(MDA)含量显著增高。(2)‘Y-14’花蕾的生长素(IAA)含量高于‘阳光玫瑰’,而脱落酸(ABA)含量显著低于‘阳光玫瑰’;‘Y-14’花蕾的赤霉素含量(GA_(3))在花蕾发育前期低于‘阳光玫瑰’,而其茉莉酸甲酯(MeJA)在后期显著低于‘阳光玫瑰’;‘Y-14’花蕾的反式玉米素核苷(TZR)含量较‘阳光玫瑰’偏低。(3)在单核早期,‘Y-14’花蕾的ABA含量变化平缓,而‘阳光玫瑰’的ABA含量急剧升高,与此同时GA_(3)含量开始高于‘阳光玫瑰’,而MeJA含量则在单核早期低于‘阳光玫瑰’且差异逐渐显著。研究认为,‘阳光玫瑰’实生后代不育株‘Y-14’花蕾发育期SOD、POD、CAT活性和MDA、IAA、ABA、GA_(3)、MeJA含量异常变化可能导致了雄性不育的发生,而且单核早期可能是‘Y-14’雄性不育产生的关键时期。

The seedling male sterile plants‘Y-14’and‘Sunshine Muscat’were used as test materials to study the changes of antioxidant enzyme activity and endogenous hormone contents during the bud development period,for demonstrating the physiological and biochemical characteristics of male sterility in grapes.The results showed that:(1)compared with‘Sunshine Muscat’,the activities of superoxide dismutase(SOD),peroxidase(POD)and catalase(CAT)in the buds of‘Y-14’were significantly reduced,while the content of malondialdehyde(MDA)increased significantly.(2)The content of auxin(IAA)in‘Y-14’was higher than that in‘Sunshine Muscat’,and the content of abscisic acid(ABA)was significantly lower than‘Sunshine Muscat’;In the early stage of bud development,the content of gibberellin(GA_(3))in‘Y-14’is lower than‘Sunshine Muscat’,while methyl jasmonate(MeJA)is significantly lower than‘Sunshine Muscat’in the later stage;the content in‘Y-14’trans-Zeatin Nucleoside(TZR)is lower than that in‘Sunshine Muscat’.(3)In the early mononuclear stage,the content of ABA in‘Y-14’changed smoothly while the‘Sunshine Muscat’increased sharply.At the same time,the content of GA_(3) began to be higher than that in‘Sunshine Muscat’,while the MeJA began to be lower than‘Sunshine Muscat’in the early mononuclear stage.And it showed gradually significant difference.In summary,the abnormal changes of SOD,POD,CAT activities and MDA,IAA,ABA,GA_(3),MeJA contents may have led to the male sterility.The early mononucleus period may be the critical period for the male sterility of‘Y-14’.