影响烟草种子产量的外源植物生长调节剂筛选

Screening of exogenous plant growth regulators affecting tobacco seed yield

以云烟87为试验材料,通过施用不同浓度及不同类型的外源植物生长调节剂,施药后调查烟株腋芽长势、开花总数、种子产量、单果粒数等,筛选出一种能够促使烟草花芽分化,增加开花总数,进而提高种子产量的植物生长调节剂类型和适宜的施用浓度。结果表明,施用多胺类(Spd、Put、Spm、Cad)、维生素E、6-BA等能明显增加烟草成花数量,其中以10 mg/L的Spd和10 mg/L的Put效果较佳,分别比对照增加44.21%和37.65%;施用Put、Spm、Cad、维生素E等能明显增加烟草种子产量,其中以30 mg/L的Put和30 mg/L的Spm效果较佳,分别比对照增加136.47%和81.18%;施用30 mg/L的Put和200 mg/L的6-BA能显著增加烟草单果粒数,分别比对照增加9.93%和4.02%;施用50 mg/L的Put和施用100 mg/L的Spd可以显著提高烟草种子的千粒重,分别比对照增加9.52%和9.90%。施用GA3、NAA对增加烟草的成花数量、种子产量和单果粒数无促进作用。

Taking Yunyan 87 as experimental material,the growth of axillary buds,the total number of flowers,the seed yield and the number of single fruit grains were investigated after applying different concentrations and different types of exogenous plant growth regulators,to select a kind of plant growth regulator which can promote tobacco flower bud differentiation,increase the total number of flowering,and then improve the seed yield and the suitable application concentration.The results showed that the application of polyamine(Spd,Put,Spm,Cad),vitamin E,6-BA and so on could increase the number of tobacco flowering obviously.Among them,10 mg/L Spd and 10 mg/L Put had the better effect,which increased by 44.21%and 37.65%,respectively.Put,Spm,Cad,vitamin E and so on could increase the yield of tobacco seed obviously.Among them,30 mg/L Put and 30 mg/L Spm had the best effect,which increased by 136.47%and 81.18%,respectively compared with the control.Application of 30 mg/L Put and 200 mg/L 6-BA significantly increased the number of single fruit grains by 9.93%and 4.02%,respectively,and application of 50 mg/L Put and 100 mg/L Spd significantly increased the 1000-grain weight of tobacco seeds,it increased by 9.52%and 9.90%,respectively.The application of GA3and NAA did not increase the number of flowers,seed yield and seed number per fruit.