金线草药材薄层鉴别及含量测定

Thin layer identification and content determination of Antenoron filiforme(Thunb.)Roberty et Vantier

采用薄层色谱法(TLC),以甲苯∶乙酸乙酯∶甲酸(10∶3∶1.5)为展开剂,展开,晾干,喷3%三氯化铝-乙醇溶液,晾干,置紫外灯(360 nm)下检视。采用高效液相色谱法,以色谱柱Eclipse XDB-C18柱(250 mm×4.6 mm,5μm),10μL进样,乙腈-0.1%的磷酸水溶液为流动相,梯度洗脱,流速1.0 mL/min,柱温30℃,检测波长360 nm,建立金线草(Antenoron filiforme)药材薄层鉴别方法以及含量测定。结果表明,对金线草药材进行薄层鉴别,斑点清洗,分离度好,能有效鉴别出金线草。同时,在30 min内完成对3种黄酮成分的分析,各成分间均达到基线分离,芦丁、槲皮素、山奈酚的线性范围分别为2.12~100.80μg/mL(r=0.9994)、25.50~758.86μg/mL(r=0.9998)、2.62~250.48μg/mL(r=0.9995),平均加样回收率(n=6)分别为98.42%、101.21%、97.92%。

Using thin layer chromatography(TLC),using toluene:ethyl acetate:formic acid(10∶3∶1.5)as the developing agent,unfold,dry,spray 3%aluminum trichloride-ethanol solution,dry,and set the UV lamp(360 nm)view.Using high performance liquid chromatography,the column Eclipse XDB-C18(250 mm×4.6 mm,5μm),10μL injection,acetonitrile-0.1%phosphoric acid aqueous solution as the mobile phase,gradient elution,flow rate was 1.0 mL/min,the temperature was 30℃,and the detection wavelength was 360 nm,establishing the thin-layer identification method and content determination method of Antenoron filiforme.Results showed that thin layer identification of Antenoron filiforme,spot cleaning,good separation,can effectively identify Antenoron filiforme.This method completed the analysis of the three flavonoid components within 30 minutes,and each component reached a baseline separation.The linear ranges of rutin,quercetin and kaempferol were 2.12~100.80μg/mL(r=0.9994),25.50~758.86μg/mL(r=0.9998),2.62~250.48μg/mL(r=0.9995),respectively.The average recovery rates(n=6)were 98.42%,101.21%,and 97.92%,respectively.