菠萝蜜白化突变体叶片表皮形态研究

Leaf Epidermal Morphology of Albino Mutant of Artocarpus heterophyllus

为探究木本植物白化突变体叶片表皮形态的变化,在扫描电镜下观测了菠萝蜜(Artocarpusheterophyllus)白化突变体(AAS)和正常(CK)幼苗叶片的表皮细胞和气孔器,对MAP65家族蛋白构建了进化树,并分析了MAP65基因的表达模式。结果表明,AAS表皮细胞和气孔器的大小、形态均发生较大变化。与CK相比,AAS表皮细胞的周长、面积较小,密度较大,凸出数量和长度均减少,气孔器较小且大小不一。下表皮小细胞和异常气孔器的数量在AAS中大幅增加。MAP65家族成员大部分基因在AAS中下调表达。因此,推测菠萝蜜白化突变体的发生可能与MAP65基因表达有关。

To investigate the changes of leaf epidermal morphology in albino mutants of woody plants,the epidermis cells and stomatal apparatus on leaves of albino mutant(AAS)and normal(CK)Artocarpus heterophyllus seedlings were observed under scanning electron microscopy.The phylogenetic tree was constructed based on MAP65 family proteins,and the expression of MAP65 was analyzed.The results showed that the size and morphology of epidermal cells and stomatal apparatus of AAS were changed greatly.Compared with CK,epidermal cells of AAS had smaller perimeter and area,higher density,less lobe number,shorter lobe length and smaller stomatal apparatus.The number of small cells and abnormal stomatal apparatus in lower epidermis increased significantly in AAS.Most genes of MAP65 family members were down regulated in AAS.Therefore,it was speculated that the occurrence of AAS might be related to the expression of MAP65 gene.