LINC01936在乳腺癌中的表达及其对细胞增殖、凋亡、迁移和侵袭特性的影响

Expression of LINC01936 in Breast Cancer and its Effect on Cell Proliferation,Apoptosis,Migration and Invasion

[目的]研究LINC01936在乳腺癌中的表达及其对细胞增殖、凋亡、迁移和侵袭的影响。[方法]从UCSC XENA数据库中获取LINC01936在1099例乳腺癌组织和292例非配对癌旁组织及112例配对样本中的表达数据。用LINC01936-pcDNA3.1或pcDNA3.1质粒转染人乳腺癌MCF-7细胞,获得过表达LINC01936的MCF-7细胞和对照MCF-7细胞。使用cell counting kit-8(CCK-8)细胞增殖实验测定LINC01936对MCF-7细胞增殖的影响。采用Transwell实验和细胞划痕实验测定细胞迁移和侵袭能力。通过细胞黏附实验检测黏附能力。荧光Hoechst33342染色法用于评价LINC01936对细胞凋亡的影响。Western blot和免疫荧光法用于检测NF-κB的蛋白水平。[结果]生物信息学分析表明,LINC01936在乳腺癌组织中低表达。CCK-8检测显示,LINC01936抑制MCF-7细胞的增殖(P<0.05)。Transwell实验及细胞划痕实验表明,转染LINC01936过表达质粒后,MCF-7细胞迁移和侵袭能力下降。细胞黏附实验表明,LINC01936减弱乳腺癌细胞的黏附能力(P<0.05)。荧光染色分析表明,LINC01936过表达促进MCF-7细胞的凋亡。Western blot和免疫荧光显示,LINC01936促进NF-κB的表达。[结论]LINC01936可能通过促进NF-κB的表达来抑制乳腺癌细胞的增殖、迁移和侵袭,并促进细胞凋亡。

[Purpose]To investigate the expression of long non-coding RNA(lncRNA)LINC01936 in breast cancer and its effect on cell proliferation,apoptosis,migration and invasion.[Methods]The data of LINC01936 expression in 1099 cases of breast cancer tissues and 292 cases of non-paired cancer adjacent tissues,as well as 112 paired samples were obtained from UCSC XENA data base.Human breast cancer MCF-7 cells were transfected with LINC01936-pcDNA3.1 or pcDNA3.1 plasmids to obtain LINC01936-overexpressed MCF-7 cells and control MCF-7 cells.CCK-8 cell proliferation assay was used to determine the effect of LINC01936 on the proliferation of MCF-7 cells.Transwell assay and cell scratch test were used to determine cell migration and invasion ability.The adhesion ability was detected by cell adhesion experiment.Fluorescence Hoechst33342 staining was used to estimate the effect of LINC01936 on cell apoptosis.Western blot and immunofluorescence were used to detect the protein level of NF-κB.[Results]Bioinformatics analysis showed that LINC01936 was poorly expressed in breast cancer tissues.CCK-8 assay showed that LINC01936 inhibited the proliferation of MCF-7 cells(P<0.05).Transwell assay and cell scratch test showed that MCF-7 cells had decreased migration and invasion ability after transfected with LINC01936 overexpression plasmid.The cell adhesion experiment showed that LINC01936 weakened the cell adhesion ability of breast cancer cells(P<0.05).Fluorescence staining assay showed that the overexpression of LINC01936 promoted apoptosis of MCF-7 cells.Western blot and immunofluorescence showed that LINC01936 promoted the expression of NF-κB.[Conclusion]LINC01936 may inhibit the proliferation,migration and invasion of breast cancer cells and promote apoptosis by promoting the expression of NF-κB.