复方藏兽药翼草景香颗粒主成分代谢机理研究

Study on metabolic mechanism of compound Tibetan medicine Yicao Jingxiang granule principal components in animal

运用PCA模型和代谢组学等方法,研究单次给药后复方藏兽药翼草景香颗粒主要成分在健康靶动物体内的变化规律及时量关系;运用主成分分析模式识别技术,寻找表征特定状态的生物标志物和潜在生物标志物的候选变量,从而揭示翼草景香颗粒在动物体内的生物学本质及代谢作用机制。选取健康西藏牦牛、绵羊,分成高、中、低剂量组,口服投喂复方藏药一次,另设不给药对照组,于给药前和给药后不同时段采集血样与粪样。利用GC-MS/MS对样本进行检测,检测数据分别通过GC-MS Postrun Analysis软件进行处理、NISI MS Search 2.0质谱库检索分析和Origin2018统计软件进行统计分析。由PCA得分可知,复方藏药中剂量组牦牛给药5.5 h、6.5 h和7.5 h时血样中藏药活性成分排名靠前,活性较高;复方藏药中剂量组绵羊给药0.5 h、7.5 h时和给药24.0 h时血样中藏药活性成分可以明显区分、得分最高。给药后牦牛、绵羊血液中检测出安息香醛、3-(2,6,6-三甲基-1-环己烯基)-2-丙烯醛、β-谷甾醇、2-己基-1-癸醇、三花生酸甘油酯、(±)-1,2-二棕榈精、2-乙基己酸乙烯酯、十二烯基丁二酸酐、二氢青蒿素等多种共有活性成分。该复方藏药发挥药效的主要成分包括安息香醛、3-(2,6,6-三甲基-1-环己烯基)-2-丙烯醛、β-谷甾醇等,这些共有成分可能与消炎、解热及抗肿瘤功效有关。

In this experiment,principal component analysis(PCA)model and metabolomics were used to study the change rule of the main components of compound Tibetan veterinary medicine Yicao Jingxiang granule in healthy target animals after single administration and the time-quantity relationship in vivo,aim to clarify the correlation between the changes of main active components in animal blood and the compatibility of drug. Pattern recognition techniques such as PCA were used to search for biomarkers and candidate variables of potential biomarkers in specific states,and to analyze the influence of metabolic network in different states,so as to reveal the biological nature and metabolic mechanism of medicine in animal. The samples were detected by GC-MS/MS,and the detection data were processed by GC-MS Postrun Analysis software,analyzed by NISI MS Search2.0 mass spectrum library retrieval analysis and Origin 2018 statistical software. According to the results of PCA scores,the contribution rate of Tibetan medicine active ingredients of yak blood samples in the medium dose group ranked the highest and showed the highest activity at different time. The active components of Tibetan medicine from sheep blood samples at 0.5 h and 7.5 h could be clearly distinguished with samples at 24 h after administration. Benzoic aldehyde,3-(2,6,6-trimethyl-1-cyclohexenyl)-2-acrolein,β-sitosterol,2-hexyl-1-decanol,triglyceride,(±)-1,2-dipalmitol,ethylene2-ethylhexanoate,dodecenylsuccinic anhydride and dihydroartemisinin were found in yak and sheep blood after administration. Benzaldehyde,3-(2,6,6-trimethyl-1-cyclohexenyl)-2-acrolein,β-sitosterol and other active ingredients are the main components of the compound Tibetan medicine,which may be related to anti-inflammatory,antipyretic and anti-tumor effects.