县级实验室应用结核分枝杆菌手工液体培养技术检测结果分析

Analysis of manual liquid culture method in county-level laboratory to detect Mycobacterium tuberculosis

收集2018年7月1日至2020年12月31日在四川省武胜县疾病预防控制中心结核病防治门诊就诊的1370例疑似肺结核患者的痰标本,分别使用手工液体培养法与中和离心固体培养法进行培养。手工液体培养法的阳性率为25.8%(353/1370),显著高于中和离心固体培养法的20.0%(274/1370)(χ^(2)=12.907,P<0.001)。手工液体培养法的报告阳性中位时间为2(2,3)周,显著短于中和离心固体培养法的3(2,4)周(Z=-9.491,P<0.001)。手工液体培养法显著提高了报告阳性率,显著缩短报告阳性时间,而且试剂成本与固体培养成本相当,适合在县级实验室应用。

Sputum specimens were collected from 1370 presumptive tuberculosis patients attending to the tuberculosis clinic of Center for Disease Control and Prevention in Wusheng County of Sichuan Province from July 1,2018 to December 31,2020.The sputum was cultured using manual liquid culture method and neutralization centrifugal solid culture method at the same time.The positive rate of the manual liquid culture method was 25.8%(353/1370),significantly higher than that of the neutralization centrifugal solid culture method(20.0%,274/1370)(χ^(2)=12.907,P<0.001).The median time to detection was 2(2,3)weeks for the manual liquid culture method,which was significantly shorter than that for the solid culture method(3(2,4)weeks,Z=-9.491,P<0.001).The manual liquid culture method significantly improved the positive rate,significantly shortened the time to detection,and was comparable in cost to solid culture,making it suitable for application in county-level laboratory.