摘要
A surface plasmon resonance imaging(SPRI)system was developed for the discrimination of proteins on a gold surface.As a label-free and high-throughput technique,SPRI enables simultaneously monitoring of the biomolecular interactions at low concentrations.We used SPRI as a label-free and parallel method to detect different proteins based on protein microarray.Bovine Serum Albumin(BSA),Casein and Immunoglobulin G(IgG)were immobilized onto the Au surface of a gold-coated glass chip as spots forming a 6×6 matrix.These proteins can be discriminated directly by changing the incident angle of light.Excellent reproducibility for label-free detection of protein molecules was achieved.This SPRI platform represents a simple and robust method for performing high-sensitivity detection of protein microarray.
基金
Supported by the National Foundation of High Technology of China(2006AA020701 and 2006AA020803)
National Program on Key Basic Research Projects 973 of China(2006CB705700)
the Nature Science Foundation of Zhejiang Province(2006C21G3210005)
Tsinghua-Yuyuan Medicine Foundation(40000510B).