香菇新品种“农香152”的选育

Breeding of New Lentinus edodes Variety of’Nongxiang 152’

针对工厂化周年栽培香菇的迫切需求,选育适合工厂化栽培的香菇新品种。以香菇101、L18、L19和SK11为亲本,采用多孢杂交技术选育新品种,并运用拮抗、ISSR、SRAP分子标记对杂交子进行鉴定。通过4种组合多孢杂交或自交共获得223个杂交子,经出菇试验,有97个杂交子能正常出菇,其中编号为Le0152杂交子的第一潮菇产量高于亲本60.2%,命名为"农香152"。农香152朵形圆整,子实体厚实致密,数量多,个头较小,产量高,出菇周期短且集中,接种至采收仅需69 d,属低温出菇型品种。经拮抗试验和ISSR、SRAP分子鉴定表明,该菌株具有分子特异性。试验采用多孢杂交的方法选育香菇是可行的,杂交选育的"农香152"具有产量高、栽培周期短等优点,适合工厂化栽培。

In response to the urgent need for factory-oriented annual cultivation of Lentinus edodes,a new variety of L.edodes suitable for factory cultivation was selected.With L.edodes strains of 101,L18,L19 and SK11 as parents,new varieties were selected by polysporic hybridization,and hybrids were identified using antagonistic,ISSR,and SRAP molecular markers.223 hybrid strains were obtained through polyspore hybridization or self-crossing of four combinations.97 hybrid strains were able to produce mushrooms normally,among which the first flush yield of the hybrid strain Le0152 was 60.2%higher than that of its parent,named’Nongxiang 152’.Nongxiang 152 is a low-temperature fruiting variety with round shape,thick and dense fruit body,large quantity,small size,high yield,short and concentrated fruiting cycle and only 69 days from inoculation to harvesting.The antagonism test and ISSR and SRAP molecular identification showed that the strain had molecular specificity.The experiment shows that it is feasible to select L.edodes by polyspora hybridization,Nongxiang 152 has the advantages of high yield and short cultivation period,which is suitable for factory cultivation.