一株野生美味扇菇菌株的驯化栽培及成分测定

Domestication and Nutritional Composition Analysis of A Wild Panellus edulis Strain

采用ITS鉴定法和形态学方法对野生美味扇菇1509菌株进行鉴定,优化其菌丝培养条件.结果表明,菌株1509最适碳源为果糖,最适氮源为蛋白胨,最适无机盐为MgSO_(4).正交试验结果表明,野生菌株1509的最适培养基配方为果糖20.0 g·L^(-1)、蛋白胨2.0 g·L^(-1)、MgSO_(4)0.75 g·L^(-1)、CaCl_(2)0.5 g·L^(-1)、KH_(2)PO_(4)1 g·L^(-1)、VB_(1)10 mg·L^(-1)、琼脂20 g·L^(-1).经人工驯化、栽培出菇获得美味扇菇子实体,测定子实体的粗多糖、粗蛋白质、钙、铁、VC、VE以及17种氨基酸含量;其中钙含量高达133.96 mg·100^(-1)g^(-1),含有7种人体必需氨基酸,必需氨基酸含量满足理想蛋白均衡值.

Wild Panellus edulis 1509 was identified by ITS identification and morphology,and its mycelium culture conditions were optimized.The results showed that the best carbon source is fructose,the best nitrogen source is peptone,the best inorganic salt is MgSO;.The orthogonal test results showed that the optimal medium formula of the wild strain 1509 is fructose 20.0 g·L^(-1),protein 2.0 g·L^(-1),MgSO_(4)0.75 g·L^(-1),CaCl_(2)0.5 g·L^(-1),KH_(2)PO_(4)1 g·L^(-1),VB_(1)10 mg·L^(-1),agar 20 g·L^(-1).P.edulis fruit bodies were obtained,and the content of crude polysaccharide,crude protein,calcium,iron,VC,VE,and 17 amino acids were determined.The calcium content is as high as 133.96 mg·100^(-1)g^(-1),contains 7 human essential amino acids,and the essential amino acid content meets the ideal protein equilibrium value.