CYP1A2基因单核苷酸多态性与前列腺增生术后尿路感染风险的关系

Association of CYP1A2 gene single nucleotide polymorphism with risk of urinary tract infection after benign prostatic hyperplasia

目的探讨细胞色素P450酶1A2(CYP1A2)单核苷酸多态性(SNP)与前列腺增生术后尿路感染易感性的关系。方法将2017年1月至2020年12月于该院就诊和治疗的行经尿道前列腺等离子双极电切术(TUPKRP)的前列腺增生患者82例纳入研究,根据是否发生尿路感染分为感染组(n=19)和非感染组(n=63)。提取2组患者外周血DNA,通过实时荧光定量PCR、聚合酶链反应-限制性片段长度多态分析(RFLP-PCR)获取CYP1A2基因SNP位点rs12558163的基因型。采用Logistic回归分析该SNP位点的基因型、等位基因,抗菌药物使用、侵入性操作是否为尿路感染的危险因素。结果CYP1A2 rs12558163位点有3种基因型(AA、AG和GG型),基因分型检测成功率100%。感染组CYP1A2 rs12558163位点AA、AG和GG基因型频率分别为73.68%、15.79%、10.53%,非感染组CYP1A2 rs12558163位点AA、AG和GG基因型频率分别为14.29%、47.62%、38.10%,感染组AA基因型频率高于非感染组(P<0.05)。以AG基因型为参考,携带CYP1A2 rs12558163 AA基因型者尿路感染风险增加(OR=1.542,95%CI:1.149~1.895,P<0.05),携带CYP1A2 rs12558163 GG基因型者尿路感染风险无明显改变(OR=1.108,95%CI:0.895~1.317,P>0.05);以AG+AA基因型为参考,携带CYP1A2 rs12558163 AA基因型者尿路感染风险增加(OR=1.488,95%CI:1.130~1.769,P<0.05)。以携带G等位基因者为参考,携带A等位基因者尿路感染风险增加(OR=1.394,95%CI:01.065~1.723,P<0.05)。多元线性回归分析结果显示,CYP1A2 rs12558163 AA基因型导致尿路感染发生的危险性大于等位基因A(P<0.05)。结论CYP1A2 rs12558163位点等位基因A可能增加前列腺增生术后尿路感染风险。

Objective To investigate the relationship between cytochrome P4501A2(CYP1A2)single nucleotide polymorphism(SNP)and susceptibility to urinary tract infection after benign prostatic hyperplasia.Methods A total of 82 patients with benign prostatic hyperplasia who underwent transurethral plasmakinetic resection of prostate(TUPKRP)from January 2017 to December 2020 in the hospital were enrolled in the study,and divided into infected group(n=19)and non-infected group(n=63)according to the presence or absence of urinary tract infection.Peripheral venous blood DNA was extracted,and genotypes at the SNP site rs12558163 of CYP1A2 gene were detected by polymerase chain reaction-restriction fragment length polymorphism(PCR-RFLP).Logistic regression was used to analyze whether the genotype and allele of the SNP locus,the use of antibiotics,and invasive procedures were risk factors for urinary tract infection.Results The CYP1A2 rs12558163 locus has 3 genotypes(AA,AG and GG),and the genotype detection success rate was 100%.The frequencies of AA,AG,and GG at CYP1A2 rs12558163 in the infected group were 73.68%,15.79%,and 10.53%,respectively,and the frequencies of AA,AG,and GG at CYP1A2 rs12558163 in the non-infected group were 14.29%,47.62%,and 38.10%,respectively.The frequency of AA in the infected group was higher than that in the non-infected group(P<0.05).Taking the AG genotype as reference,the risk of urinary tract infection increased in CYP1A2 rs12558163 AA genotype carriers(OR=1.542,95%CI:1.149—1.895,P<0.05),while the risk of urinary tract infection did′t significantly changed in CYP1A2 rs12558163 GG genotype carriers(OR=1.108,95%CI:0.895—1.317,P>0.05);taking the AG+AA genotype reference,the risk of urinary tract infection increased in the CYP1A2 rs12558163 AA genotype carriers(OR=1.488,95%CI:1.130—1.769,P<0.05).Taking the G allele as reference,the risk of urinary tract infection increased in the A allele carriers(OR=1.394,95%CI:01.065—1.723,P<0.05).The results of multiple linear regression analysis showed that the risk of urinary tract infection caused by CYP1A2 rs12558163 locus AA gene was higher than that of allele A(P<0.05).Conclusion Allele A of CYP1A2 rs12558163 may be associated with the susceptibility of urinary tract infection after TUPKRP.