急性缺血性脑卒中患者血清lncRNA SNHG12表达水平检测及临床意义

Detection of Serum lncRNA SNHG12 Expression Level in Patients with Acute Ischemic Stroke and Its Clinical Significance

目的 通过检测急性缺血性脑卒中患者血清长链非编码RNA小核仁RNA宿主基因12(long non-coding RNA small nucleolar RNA host gene 12,lncRNA SNHG12)表达变化情况,探究其在急性缺血性脑卒中的临床意义。方法 选取2019年3月~2021年2月秦皇岛市第一医院神经内科收治的95例急性缺血性脑卒中(acute ischemic stroke, AIS)患者作为研究组,根据美国国立卫生研究院卒中量表(National Institute of Health Stroke Scale,NIHSS)评分将患者分为轻度组29例、中度组36例、重度组30例;选取同期医院健康体检者72例作为对照组;实时荧光定量PCR法检测研究组患者入院24 h内、治疗1周后、治疗2周后以及对照组体检当日血清lncRNA SNHG12表达情况;采用Pearson相关系数法分析急性缺血性脑卒中患者血清lncRNA SNHG12表达水平与NIHSS评分的相关性;受试者工作特征曲线评估血清lncRNA SNHG12表达水平对急性缺血性脑卒中的诊断价值。结果 与对照组比较,入院24 h内轻度组、中度组、重度组血清lncRNA SNHG12表达水平依次升高(1.29±0.17,1.52±0.26,1.80±0.43 vs 1.01±0.12),差异有统计学意义(F=68.522,P=0.000);研究组患者入院24 h内、治疗1周后、治疗2周后血清lncRNA SNHG12表达水平依次降低(1.45±0.25,1.30±0.24,1.18±0.17),差异有统计学意义(F=35.003,P=0.000);研究组患者入院24h内血清lncRNA SNHG12表达水平与NIHSS评分呈正相关(r=0.358,P=0.000);入院24 h内血清lncRNA SNHG12表达水平评估急性缺血性脑卒中发生的曲线下面积为0.953(95%CI:0.923~0.982),敏感度和特异度分别为86.30%,95.80%。结论 急性缺血性脑卒中患者血清lncRNA SNHG12表达水平显著升高,和患者疾病严重程度相关,对急性缺血性脑卒中有较高的诊断价值。

Objective To explore the changes in the expression of long non-coding RNA small nucleolar RNA host gene 12(lncRNA SNHG12) in the serum of patients with acute ischemic stroke(AIS), and explore its clinical significance in acute ischemic stroke. Methods From March 2019 to February 2021, 95 patients with acute ischemic stroke to the department of neurology, the First Hospital of Qinhuangdao were selected as the study group, according to National Institute of Health Stroke Scale(NIHSS) score, the patients were divided into mild group 29 cases, moderate group 36 cases and severe group 30 cases. 72healthy people who had physical examination in the hospital during the same period served as the control group. The expression of serum lncRNA SNHG12 in the study group was detected by real-time fluorescent quantitative PCR within 24 hours after admission, 1 week after treatment, 2 weeks after treatment and on the day of physical examination in the control group;Pearson correlation coefficient method was used to analyze the correlation between serum lncRNA SNHG12 expression level and NIHSS score of patients with acute ischemic stroke. Receiver operating characteristic curve was used to assess the value of serum lncRNA SNHG12 expression in samples for the diagnosis of acute ischemic stroke. Results Compared with the control group,the expression level of serum lncRNA SNHG12 in mild group, moderate group and severe group were increased in turn within 24hours after admission(1.29±0.17, 1.52±0.26, 1.80±0.43 vs 1.01±0.12),the difference was statistically significant(F=68.522,P=0.000). The expression levels of serum lncRNA SNHG12 in the study group decreased in turn within 24 hours after admission,1 week after treatment and 2 weeks after treatment(1.45±0.25, 1.30±0.24, 1.18±0.17), the difference was statistically significant(F=35.003, P=0.000). The expression level of serum lncRNA SNHG12 was positively correlated with NIHSS score in the study group within 24 hours after admission(r=0.358, P=0.000). The area under the curve of serum lncRNA SNHG12expression level within 24 hours after admission in evaluating the occurrence of acute ischemic stroke was 0.953(95% CI:0.923~0.982), the sensitivity and specificity were 86.30% and 95.80%, respectively. Conclusion The expression level of serum lncRNA SNHG12 in patients with acute ischemic stroke was significantly increased, which was related to the severity of the disease, and has high diagnostic value for acute ischemic stroke.