CCL2(rs3760399)基因多态性和前列腺癌临床病理特征的相关性

Correlation between CCL2(rs3760399)gene polymorphism and clinicopathological characteristics of prostate cancer

目的探讨CCL2(rs3760399)基因多态性和前列腺癌临床病理特征之间的相关性,同时比较比较本中心人群与其他人种CCL2基因变异频率的差异。方法回顾性分析2014年6月至2019年6月复旦大学附属肿瘤医院泌尿外科收治的182个前列腺癌患者的临床资料。182例患者均行DNA测序得到CCL2的基因型。根据基因检测结果不同将患者分为变异组(AG/GG)和野生组(AA)。同时纳入一篇分析1765个来自美国高加索人的文献。采用非配对的t检验和χ;检验分析CCL2基因型和Gleason评分、临床M分期和转移负荷等前列腺癌病理特征之间的相关性,同时比较本中心和美国高加索人群CCL2变异频率的差异。结果本中心的182例患者中,CCL2基因型为AG/GG的变异组患者33例(18.1%),基因型为AA的野生组患者149例(81.9%)。纳入文献中美国高加索人群的变异组患者占比为6.8%(120/1765),变异频率低于本中心(18.1%),差异具有统计学意义(P<0.001)。在基因型与临床病理特征的探索性分析中,野生组中高转移负荷者69例,低转移负荷38例,变异组中高转移负荷者7例,低转移负荷者12例,野生组中的高转移负荷者多于变异组,差异具有统计学意义(P=0.023)。结论CCL2基因型与患者转移负荷状态具有相关性,野生组患者中高转移负荷患者的比例高于变异组,支持了CCL2变异对于前列腺癌进展的影响;同时,本中心CCL2基因变异的频率与美国高加索人不同,前者变异频率较后者高,理论上对于预测疗效的价值更大。

Objective To investigate the correlation between CCL2(rs3760399)gene polymorphism and clinicopathological features of prostate cancer,and to compare the variation frequency of CCL2 gene between our center and other ethnic groups.Methods Clinical data of 182 prostate cancer patients admitted to the Department of Urology,Shanghai Cancer Center from June 2014 to June 2019 were retrospectively analyzed.All the 182 patients underwent DNA sequencing and obtained the genotype of CCL2.The patients were divided into variant group(AG/GG)and normal group(AA)according to the genetic test results.A review involved 1765 Caucasians from United States was also included.Unpaired t test and Chi-square test were used to analyze the correlation between CCL2 genotype and Gleason score,clinical M stage,and metastatic burden of prostate cancer,and to compare the variation frequency of CCL2 between our center and Caucasian patients.Results Among the 182 patients in our center,33 patients(18.1%)were in the variant group of AG/GG genotype,and 149 patients(81.9%)were in the wide-type group of AA genotype.In the included literature,the proportion of Caucasian patients in the variation group was 6.8%(120/1765),which was 18.1%lower than that in our centre(P<0.001),and the difference was statistically significant.In the exploratory analysis of genotype and clinicopathological characteristics,69 cases of high and 38 cases of low metastatic load were found in the wide-type group,7 cases of high and 12 cases of low metastatic load were found in the variation group,and the number of high metastatic load in the wide-type group was more than that in the variation group(P=0.023),the difference was statistically significant.Conclusion CCL2 genotype is correlated with the status of patients with metastatic load.The proportion of patients with high metastatic load in the wide-type group is higher than that in the variant group,which supports the influence of CCL2 mutation on the progression of prostate cancer.Meanwhile,the frequency of CCL2 gene variation in our centre patients is different from that in the Caucasian population.The frequency of CCL2 gene variation in the Chinese population is higher than that in the Caucasian population,which is theoretically more valuable for predicting efficacy.