枣仁茯苓玉竹膏改善阴虚型失眠作用研究

ZaoRenFuLingYuZhu plasters for Study on the effect of improving insomnia of yin deficiency type

目的 通过戊巴比妥钠阈下催眠剂量试验和复制阴虚型失眠动物模型,探讨枣仁茯苓玉竹膏对阴虚型失眠的改善作用。方法 戊巴比妥钠阈下催眠剂量试验,计算每组小鼠的入睡率。用甲状腺素复制阴虚型失眠大鼠模型。监测各组大鼠体质量、饮水量、进食量、肛温;旷场实验检测行为学变化;ELISA检测血清中5-HT、DA、NE、γ-GABA、Glu、TSH,血浆中cAMP、cGMP含量;Western Blot检测脑组织中CRH和ACTH表达水平。结果 戊巴比妥钠阈下催眠剂量试验结果表明,枣仁茯苓玉竹膏能增加小鼠入睡率。阴虚型失眠动物模型结果表明,枣仁茯苓玉竹膏可以明显改善由甲状腺素诱导的阴虚型失眠动物外观体征,行为学,5-HT、DA、NE、γ-GABA、Glu、TSH,血浆中cAMP、c GMP含量及脑组织中CRH和ACTH表达水平(P<0.05或P<0.01)。结论 枣仁茯苓玉竹膏可以改善由甲状腺素所致的阴虚型失眠模型大鼠的失眠症状,其作用机制可能与调节5-HT、DA、NE、γ-GABA、Glu、c AMP、cGMP、TSH、CRH和ACTH含量有关。

Objective To explore the improvement effect of Zaoren Fuling Yuzhu plasters(ZFYP)on insomnia of yin deficiency type by subthreshold hypnotic dose test of pentobarbital sodium and reproduction of insomnia animal model of yin deficiency type.Method Subthreshold hypnotic dose test of pentobarbital sodium was used to calculate the sleep rate of mice in each group.The insomnia rat model of yin deficiency type was reproduced with thyroxine.Body weight,water intake,food intake and Anal temperature were monitored;open field experiment was used to detect behavioral changes;ELISA was used to detect 5-HT,Da,NE in serumγ-GABA,Glu,TSH,camp and cGMP in plasma were detected;the expression levels of CRH and ACTH were detected by Western blot.Results The results of subthreshold hypnotic dose test of pentobarbital sodium showed that ZFYP could significantly reduce the number of spontaneous activities and increase the sleep rate of mice.The animal model of insomnia of yin deficiency type was established,the results showed that compared with the model group,ZFYP could significantly improve the appearance,behavior,5-HT,DA,NE,γ-GABA,Glu,TSH,cAMP,cGMP in plasma and CRH and ACTH in brain tissue(P<0.05 or P<0.01)with Yin deficiency insomnia induced by thyroxine.Conclusion ZFYP can improve the insomnia symptoms of yin deficiency insomnia model rats induced by thyroxine,and its mechanism may be related to the regulation of 5-HT,Da,NEγ-The contents of GABA,Glu,camp,cGMP,TSH,CRH and ACTH.