光学透明技术在植物多尺度成像中的应用

Applications of Optical Clearing Technique in Multi-scale Imaging

光学透明技术是一种通过各种化学试剂,将原本不透明的生物样本实现透明化,并在光学显微镜下深度成像的技术。结合多种光学显微成像新技术,光学透明技术可对整个组织进行成像和三维重建,深度剖析生物体内部空间特征与形成机制。近年来,多种植物光学透明技术和多尺度成像技术被陆续研发,并取得了丰硕的研究成果。该文综述了生物体光学透明技术的基本原理和一些新技术,重点介绍基于光学透明技术开发的新型成像方法及其在植物成像与细胞生物学中的应用,为后续植物整体、组织或器官的透明、成像与三维重构及功能研究提供理论依据和技术支持。

The optical clearing techniques are common methods that allow biological tissues or organs to be deeply transparent and imaged under an optical microscope by processing with chemical reagents. Driven by a variety of optical microscopy technologies, the entire tissue can be imaged and reconstructed three-dimensionally(3D) using the clearing technique to deeply analyze the internal spatial characteristics and formation mechanism of biological tissues. In recent years, several optical clearing techniques and multi-scale imaging techniques have been developed and fruitful researches have been reported. This article discusses basic principles and some new techniques of optical clearing, with particular focus on new imaging methods based on optical clearing technique and their applications in plant imaging and cell biology. This review will provide theoretical basis and technical support for the subsequent research on clearing, imaging and 3D reconstruction and function of whole plants, tissues or organs.