脓毒症早期BMP-2和BMP-4的变化

Changes of BMP-2 and BMP-4 in early phase of sepsis

目的探讨骨形态发生蛋白-2(BMP-2)和骨形态发生蛋白-4(BMP-4)在脓毒症发病中的变化规律及作用机制。方法收集入住重症医学科(ICU)24 h内的脓毒症患者(脓毒症组)、非脓毒症患者(非脓毒症组)和健康志愿者(对照组)的血清,ELISA测定血清BMP-2和BMP-4浓度。免疫印迹法检测经脂多糖(LPS)处理后的大鼠肺微血管内皮细胞(PMECs)的BMP-2、磷酸化Smad(pSmad)1/5和BMP受体Ⅱ(BMPRⅡ)的表达。结果脓毒症患者血清BMP-2和BMP-4水平显著较高(P<0.05);脓毒症组BMP-2水平显著高于非脓毒症组(P<0.05)。BMP-2在脓毒症和健康志愿者间及脓毒症和非脓毒症组间有较好的诊断价值。LPS可显著升高PMECs的BMP-2表达量,而降低pSmad1/5和BMPRⅡ的表达量(P<0.05)。结论BMP-2可能是脓毒症早期潜在的诊断标志物,并作为促炎因子起作用。

Objective To study the change of serum bone morphogenetic protein(BMP)-2 and BMP-4 during the development of sepsis and its mechanisms.Methods Blood was collected for quantifying serum BMP-2 and BMP-4 from patients with sepsis(sepsis group),patients without sepsis(non-sepsis group)in 24 hours of ICU admission and from healthy volunteers(control group).Concentration of serum BMP-2 and BMP-4 was determined by ELISA.The expression of BMP-2,pSmad1/5,and BMPRⅡof lip polysaccharide(LPS)-treated rat pulmonary microvascular endothelial cells(RPMECs)was detected by Western blot.Results Serum BMP-2 and BMP-4 were significantly increased in sepsis group(P<0.05)and the level of BMP-2 was significantly higher in sepsis group than that in non-sepsis group(P<0.05).BMP-2 was found to be a marker for differential diagnosis of separating sepsis LPS may significantly increase the expression of BMP-2 of RPMECs but decrease the expression of phosphorylated Smad1/5(pSmad1/5)and BMP receptorⅡ(BMPRⅡ)(P<0.05).ConclusionsBMP-2 is a potential diagnostic marker and act as apro-inflammatory factor in early phase of sepsis.