儿童原发免疫性血小板减少症miR-106b-5p的表达及其与T细胞的相关性研究

Expression of miR-106b-5p in children with primary immune thrombocytopenia and its correlation with T cells

目的 探讨血浆miR-106b-5p在原发免疫性血小板减少症(primary immune thrombocytopenia,ITP)中的表达及其与辅助性T细胞17(T helper cells 17,Th17)、调节性T细胞(T regulatory cell,Treg)、Th17/Treg的相关性。方法 选取79例ITP患儿(ITP组)和40例健康儿童(对照组)为研究对象。79例ITP患儿根据治疗效果分为完全有效组(40例)、有效组(18例)、无效组(21例)。采用实时荧光定量PCR技术检测miR-106b-5p表达水平,流式细胞技术检测Th17和Treg,计算Th17/Treg,分析血浆miR-106b-5p表达水平与Th17、Treg及Th17/Treg的相关性。结果 ITP组miR-106b-5p、Th17及Th17/Treg水平高于对照组(P<0.05),Treg水平低于对照组(P<0.05)。ITP组患儿治疗后miR-106b-5p、Th17、Th17/Treg水平低于治疗前(P<0.05),Treg水平高于治疗前(P<0.05)。完全有效组miR-106b-5p、Th17、Th17/Treg水平低于有效组和无效组(P<0.05),Treg水平高于有效组和无效组(P<0.05)。相关分析显示,ITP患儿血浆miR-106b-5p表达水平与Th17、Th17/Treg均呈正相关(分别r=0.730、0.816,均P<0.001),与Treg呈负相关(r=-0.774,P<0.001)。结论 ITP患儿存在miR-106b-5p高表达和Th17/Treg比例失衡,在ITP患儿治疗过程中检测miR-106b-5p、Th17、Treg及Th17/Treg水平,对ITP患儿的疗效判断具有较好的指导作用。

Objective To study the expression level of plasma miR-106b-5p in primary immune thrombocytopenia(ITP) and its correlation with the levels of T helper 17 cell(Th17) and regulatory T cell(Treg) and the Th17/Treg ratio.Methods A total of 79 children with ITP(ITP group) and 40 healthy children(control group) were selected as subjects.According to the treatment response,the 79 children with ITP were divided into three groups:complete response(n=40),partial response(n=18),and non-response(n=21).Quantitative real-time PCR was used to measure the expression level of miR-106b-5p.Flow cytometry was used to measure the frequencies of Th17 and Treg,and the Th17/Treg ratio was calculated.The correlation of the expression level of plasma miR-106b-5p with the frequencies of Th17 and Treg and the Th17/Treg ratio was analyzed.Results Compared with the control group,the ITP group had significantly higher levels of miR-106b-5p,Th17,and Th17/Treg ratio(P<0.05) and a significantly lower level of Treg(P<0.05).After treatment,the ITP group had significant reductions in the levels of miR-106b-5p,Th17,and Th17/Treg ratio(P<0.05) and a significant increase in the level of Treg(P<0.05).Compared with the partial response and non-response groups,the complete response group had significantly lower levels of miR-106b-5p,Th17,and Th17/Treg ratio(P<0.05) and a significantly higher level of Treg(P<0.05).The correlation analysis showed that in the children with ITP,the expression level of plasma miR-106b-5p was positively correlated with the Th17 level and the Th17/Treg ratio(r=0.730 and 0.816respectively;P<0.001) and was negatively correlated with the Treg level(r=-0.774,P<0.001).Conclusions A higher expression level of miR-106b-5p and Th17/Treg imbalance may be observed in children with ITP.The measurement of miR-106b-5p,Th17,Treg,and Th17/Treg ratio during treatment may be useful to the evaluation of treatment outcome in children with ITP.