多光子技术可视化血管型脑膜瘤

Multiphoton Technique for Visualization of Angiomatous Meningiomas

血管型脑膜瘤血管异常丰富。术前或者术中能明确诊断血管型脑膜瘤对手术方案的制定和预后判断至关重要。联合二次谐波技术、双光子激发荧光技术和光谱技术无标记地对血管型脑膜瘤进行研究。结果表明,多光子技术不仅可以识别血管型脑膜瘤的内源成分,还能可视化它的微结构,联合图像分析技术,还可以进一步自动定位血管的位置并计算血管的数量。随着光纤技术和多光子内窥镜技术的发展,多光子技术有望成为临床诊断血管型脑膜瘤的新技术。

Angiomatous meningiomas comprise numerous blood vessels.The ability to clearly diagnose angiomatous meningiomas preoperatively or intraoperatively is crucial for developing surgical plans and prognosis.In this study,we combined the second harmonic,two-photon excited fluorescence,and spectroscopic techniques to realize a label-free study of angiomatous meningiomas.Results show that this multiphoton technique can identify the endogenous components of angiomatous meningioma and visualize its microstructure.Furthermore,combined with image analysis techniques,the multiphoton technique can further automatically locate vessels and calculate number of vessels.With the rapid development in fiber optic and multiphoton endoscopic technologies,multiphoton technique poses as a new technique for the clinical diagnosis of angiomatous meningiomas.