HLLA1基因单核苷酸多态性与高发区食管鳞癌患者术后生存分析

HLLA1 SNP and Prognosis of Patients with Esophageal Squamous Cell Carcinoma in High Incidence Area

目的探究单核苷酸多态性(SNP)与高发区食管鳞癌患者预后生存的关系。方法采用全外显子测序技术得到与高发区食管鳞癌患者相关的多态性目标SNP(rs2280851),用液相色谱质谱法获得相应蛋白表达量,将目标SNP(rs2280851)与患者术后生存用软件SPSS 17.0进行Kaplan-Meier法,Log-rank检验进行生存期分析,找到目标SNP(rs2280851)在NCBI数据库中对应的基因,将目标SNP(rs2280851)与相应蛋白表达量进行相关分析。结果rs2280851位点突变型G/G对食管鳞癌患者的生存影响较大(P<0.05),是可疑致病SNP;rs2280851位点在NCBI数据库中与HLLA1基因相关;rs2280851的SNP多态性与相关联CTR9蛋白的表达呈负相关。结论rs2280851位点突变型G/G是可疑致病SNP,通过调控HLLA1基因,降低表达CTR9,从而显著降低高发区食管癌患者的术后生存期。

Objective To explore the relationship between SNP and prognosis and survival in patients with esophageal squamous cell carcinoma in high incidence area.Methods The polymorphism target SNP(rs2280851)associated with high-incidence esophageal squamous cell carcinoma was obtained by whole exon sequencing technology,and the corresponding protein expression was obtained by liquid chromatography mass spectrometry.Kaplan-meier method and log-rank test were used to analyze the survival of target SNP(rs2280851)and postoperative survival of patients with SPSS 17.0 software,and the corresponding gene of target SNP(rs2280851)in NCBI database was found.Correlation analysis was performed between the target SNP(rs2280851)and the corresponding protein expression.Results rs2280851 mutant G/G is a suspected pathogenic SNP,and the postoperative survival of patients with esophageal cancer in high incidence area can be significantly reduced by regulating HLLA1 gene and reducing CTR9 expression.Conclusion rs2280851 mutant G/G had a significant effect on the survival of patients with esophageal squamous cell carcinoma,P<0.05,indicating that it was a suspected pathogenic SNP.rs2280851 was correlated with HLLA1 gene in NCBI database.SNP polymorphism of rs2280851 was negatively correlated with the expression of associated CTR9 protein.