土壤中抑菌放线菌的筛选鉴定及发酵条件研究

Study on Screening and Identification of Bacteriostatic Actinomycetes in Soil and Fermentation Conditions

采用弹土法从高黎贡山百花岭段土壤中共分离到放线菌38株,对分离得到的38株放线菌的发酵液进行抑菌活性测定,其中H33菌株对枯草芽孢杆菌、金黄色葡萄球菌和白假丝酵母都具有较好的抑菌作用,其抑菌直径都在8 mm以上。通过对H33菌株培养特征、显微特征和生理生化特征的研究,初步鉴定为链霉菌属(Streptomyces)。H33菌株的最佳发酵培养基为发酵培养基2(葡萄糖0.5 g、蛋白胨0.25 g、胰胨0.15 g、NaCl 0.25 g、重铬酸钾7.5 mg、复合维生素10 g、水500 mL,pH 7.2),最佳发酵时间为6 d,最适发酵培养基pH为7.0,最适培养温度28℃。

A total of 38 actinomycetes were isolated from the soil in Baihualing section of Gaoligong Mountain by the bombing method,and the antibacterial activity of the 38 isolated actinomycetes was tested.Among them,the H33 strain had good antibacterial effects on Bacillus subtilis,Staphylococcus aureus and Candida albicans,and its antibacterial diameter was all above 8 mm.Through the study of the culture characteristics,microscopic characteristics and physiological and biochemical characteristics of the H33 strain,it was preliminarily identified as Streptomyces.The best fermentation medium for H33 strain was fermentation medium 2(glucose 0.5 g,peptone 0.25 g,tryptone 0.15 g,NaCl 0.25 g,potassium dichromate 7.5 mg,multivitamin 10 g,water 500 mL,pH 7.2),the optimal fermentation time was 6 d,the optimal fermentation medium pH was 7.0,and the optimal culture temperature was 28℃.