CDC45在肝细胞癌组织中的表达及其与预后的相关性

Expression of CDC45 in hepatocellular carcinoma and its correlation with prognosis

目的分析细胞分化周期蛋白45(CDC45)在肝细胞癌(HCC)组织中的表达情况,探讨CDC45与HCC预后的关系,对CDC45作为HCC的潜在生物标志物和治疗靶点的可能性进行初步探究。方法采用生物信息学方法分析CDC45在HCC组织及癌旁正常组织中的mRNA水平,并分析其表达与HCC患者的生存率间的关系。回顾性分析90例接受手术治疗的HCC患者的临床病理资料。采用免疫组化法检测HCC组织和癌旁正常组织中的CDC45表达水平,并分析其与HCC患者临床病理特征的关系。结果CDC45在结直肠癌、乳腺癌、前列腺癌、肺癌、肝癌等多种肿瘤中高表达。CDC45在HCC组织中的表达量高于癌旁正常组织中的表达量(P<0.05)。CDC45的表达与HCC患者的临床预后显著相关(P<0.001),且CDC45的表达量越高,HCC患者的无病生存率和总生存率越低。CDC45与HCC患者的肿瘤结节数(P=0.003)及肿瘤大小(P=0.003)显著相关,而与年龄、性别和甲胎蛋白(AFP)水平的相关性无统计学意义(均P>0.05)。结论CDC45在HCC的进展中扮演着重要的角色,有望成为HCC的治疗靶点和潜在生物标志物。

Objective To analyze the expression of cell differentiation cyclin 45(CDC45)in hepatocellular carcinoma(HCC)tissues,to explore the relationship between CDC45 and the prognosis of HCC,and to explore the possibility of CDC45 as a potential biomarker and therapeutic target for HCC.Methods Bioinformatics methods were used to analyze the mRNA levels of CDC45 in HCC tissues and normal tissues,and to analyze the relationship between its expression and the survival rate of HCC patients.The clinicopathological data of 90 patients with HCC undergoing surgical treatment were retrospectively analyzed.Immunohistochemical method was used to detect the expression level of CDC45 protein in HCC tissue and normal tissues,and to analyze its relationship with clinicopathological characteristics of patients with HCC.Results CDC45 is highly expressed in colorectal cancer,breast cancer,prostate cancer,lung cancer,liver cancer,etc.The expression of CDC45 in HCC tissues was higher than that in normal tissues adjacent to cancer(P<0.05).The expression of CDC45 is significantly related to the clinical prognosis of HCC patients(P<0.001),and the higher the expression of CDC45,the lower the disease-free survival rate and overall survival rate of HCC patients.CDC45 level is significantly correlated with the number of tumor nodules(P=0.003)and tumor size(P=0.003)in HCC patients,but the correlation between CDC45 level and age,gender and alpha-fetoprotein(AFP)levels is not statistically significant(all P>0.05).Conclusions CDC45 plays an important role in the progression of HCC and is expected to become a therapeutic target and potential biomarker for HCC.