利用血清筛选和模拟胃肠液消化稳定性试验评价植物源重组人血清白蛋白潜在致敏性

Evaluation of the potential allergenicity of Oryza sativa recombinant human serum albumin by serum screening and simulated gastrointestinal fluid digestion stability test

目的利用血清筛选试验和模拟胃肠液消化稳定性试验,探讨植物源重组人血清白蛋白(OsrHSA)是否具有潜在致敏性。方法选择对虾、小麦、屋尘螨、鸡蛋、牛奶过敏,特异性IgE抗体浓度大于3.5 kUA/L的患者血清75份和健康人血清4份,利用酶联免疫吸附试验探讨OsrHSA能否与过敏血清中的过敏原特异性IgE抗体结合;按照国家标准《转基因生物及其产品食用安全检测模拟胃肠液外源蛋白质消化稳定性试验方法》进行OsrHSA的模拟胃肠液消化稳定性试验,评估OsrHSA的消化稳定性。结果有3份对牛奶过敏的血清与OsrHSA发生免疫交叉反应,该蛋白与牛奶过敏原可能存在抗原交叉性;OsrHSA在模拟胃肠液中15 s内即被消化完全,表明该蛋白极易被消化。结论OsrHSA的潜在致敏性较低,对牛奶过敏的人群需要慎重使用。

Objective Using serum screening test and simulated gastrointestinal fluid digestion stability test to explore whether Oryza sativa recombinant human serum albumin(OsrHSA)has potential allergenicity.Methods In this study,75 serum samples from patients with allergies to shrimp,wheat,house dust mites,eggs and milk,which containing specific IgE antibody concentrations greater than 3.5 kUA/L,as well as 4 healthy human serum samples were selected.Enzyme-linked immunosorbent assay was used to investigate whether OsrHSA could bind to allergen-specific IgE antibodies in the serum.The digestive stability test of the simulated gastrointestinal fluid of OsrHSA protein was carried out in accordance with the national food safety standard,detection of genetically modified organisms and derived products-simulated gastrointestinal fluid digestion stability test.Results Three sera allergic to milk had immunological cross-reactions with OsrHSA,indicating that the protein might have antigenic intersection with milk allergens.OsrHSA was completely digested within 15 s in the simulated gastric and intestinal fluid,indicating that the protein was easily digested.Conclusion OsrHSA has low potential allergenicity and people who are allergic to milk needs consideration.