超高效液相色谱-串联质谱法测定鱼肉组织中9种黄色染色剂

Determination of 9 yellow colorants in fish tissues by ultra performance liquid chromatography-tandem mass spectrometry

目的建立超高效液相色谱-串联质谱法(UPLC-MS/MS)正负离子切换模式同时测定鱼肉组织中9种黄色染色剂的分析方法。方法试样经氨水甲醇溶液和甲酸水甲醇溶液提取,上清液旋蒸复溶,正己烷除脂,离心后上清液用UPLC-MS/MS法测定,外标法定量。结果柠檬黄、日落黄、喹啉黄在2~30μg/kg,酸性橙Ⅱ、碱性橙2、碱性橙21、碱性橙22、硫黄素T、碱性嫩黄O在0.2~3.0μg/kg范围内线性关系良好,检出限分别为2μg/kg和0.2μg/kg,回收率在80.03%~107.06%,相对标准偏差在1.2%~6.6%。结论本方法适用于鱼肉组织中9种黄色染色剂的测定。

Objective To establish a detection method for 9 yellow colorants in fish tissues by ultra performance liquid chromatography-tandem mass spectrometry(UPLC-MS/MS).Methods Sample was extracted by ammonia methanol solution and formic acid water methanol solution,the supernatant was evaporated and redissolved,the fat was removed by n-hexane,determinated by UPLC-MS/MS method after centrifugation,and quantification was based on external calibration.Results The result showed good linearity in the ranges of 2-30μg/kg for tartrazine,sunset yellow and quinoline yellow,0.2-3.0μg/kg for acid orangeⅡ,basic orange 2,basic orange 21,basic orange 22,thioflavin T and auramine O,and the limits of detection were 2μg/kg and 0.2μg/kg.The recoveries were 80.03%-107.06%and the relative standard deviations(n=6)were 1.2%-6.6%.Conclusion The method can be applied to determine 9 yellow colorants in fish tissues.