智三针对血管性痴呆小鼠前额叶和海马小清蛋白、生长激素抑制素、神经肽Y神经元表达的影响

Effects of Zhisan Acupuncture on Expression of Parvalbumin(PV),Neuropeptide Y(NPY)and Somatostain within Neurons in Prefrontal Cortex and Hippocampus of Vascular Dementia Mice

【目的】探讨智三针治疗血管性痴呆(VD)小鼠的可能机制。【方法】从40只雄性C57BL6J小鼠中随机选取8只作为假手术组,其余进行造模。采用动脉夹可逆性夹闭双侧颈总动脉法建立VD小鼠模型,将造模成功的小鼠随机分为智三针组、非穴组和模型组,每组各10只。造模成功后,开始进行每日针刺治疗,连续28 d。以Y迷宫和新物体实验检测小鼠治疗后认知记忆能力改善情况,采用免疫荧光染色法检测小鼠前额叶和海马CA1区的小清蛋白(PV)、生长激素抑制素(SST)和神经肽Y(NPY)神经元的表达情况。【结果】与模型组比较,智三针组的Y迷宫的自发交替率提高(P<0.05),新物体辨别系数增加(P<0.05)。免疫荧光染色结果显示,与模型组比较,智三针组的前额叶和海马CA1区PV、NPY神经元表达量显著增加(P<0.05)。【结论】电针智三针穴干预可以改善VD模型小鼠的认知记忆能力,其机制可能与提高前额叶和海马CA1区PV、NPY神经元表达量,改善神经元损伤有关。

Objective To explore the possible mechanisms of Zhisan acupuncture treatment for VD.Methods Eight mice were randomly selected from forty male C57 BL6 J mice as the sham-operated group,and the rest were modeled.The VD model was established by arterial-clamps reversible clamping of the bilateral common carotid arteries in mice.The successfully modeled mice were randomly divided into the Zhisan acupuncture group,the non-acupuncture group and the model group,with 10 mice in each group.After successful modeling,daily acupuncture treatment was performed for 28 consecutive days.The mice were tested for improvement in cognitive memory after treatment by Y-maze and new object tests.The expression of parvalbumin(PV),somatostain(SST)and neuropeptide Y(NPY)neurons in the prefrontal regions and hippocampus CA1 area of the mice was measured by immunofluorescence staining.Results Compared with model group,both percentage of spontaneous alternation in Y maze in Zhisan acupuncture acupuncture group and new object discrimination coefficient were increased(P<0.05).The results of immunofluorescence staining showed that the expression of PV and NPY neurons in prefrontal lobe and hippocampal CA1 area of Zhisan acupuncture group was significantly increased(P<0.05)compared with the model group.Conclusion The electroacupuncture intervention of the Zhisan acupuncture points has effect in improving the cognitive memory abilities of VD model mice,and the mechanism may be related to increasing the expression of PV and NPY neurons in the prefrontal and hippocampal CA1 region and thus improving neuronal damage.