围绝经期女性超重、胰岛素抵抗与肠道菌群的相关性分析

目的分析围绝经期女性超重与相关血液指标和肠道菌群的相关性。方法选择2017年12月至2019年12月围绝经期患者72例,分为体重正常组(正常组)和体重超重组(超重组),并检测两组志愿者血脂、血糖、空腹胰岛素等血液指标,计算胰岛素抵抗指数(HOMA-IR),收集大便样本,并应用16S rRNA高通量测序分析肠道菌群结构与多样性。结果超重组甘油三酯、总胆固醇、低密度脂蛋白胆固醇(LDL)、高密度脂蛋白胆固醇(HDL)和空腹血糖与正常组比较,差异无统计学意义(P>0.05);超重组空腹胰岛素水平及HOMA-IR高于正常组,而差异有统计学意义(P<0.01)。在门水平中厚壁菌门、拟杆菌门是围绝经期女性肠道微生物群落中最丰富的菌群。超重组围绝经期女性肠道微生物多样性与正常组比较差异无统计学意义(P>0.05),但在纲水平上,超重组变形菌数量高于正常组患者(P<0.01),在科和目水平上脱硫弧菌数量显著高于正常组患者(P<0.01)。结论围绝经期女性体重增加的同时伴随胰岛素抵抗的产生,可能与肠道菌群结构改变有关。

Objective To analyze the correlation of the overweight and related blood indicates and intestinal flora in perimenopausal women.Methods A total of 72 perimenopausal women were divided into normal weight group(normal group)and overweight group according to body mass index(BMI).Blood samples were collected to detect blood lipid,blood glucose,fasting insulin blood indexes,and calculate HOMA-IR.Stool samples were coUected to analyze the structure of gut microbiota by 16S rRNA amplicon sequencing.Results Compared with the normal group,the triglyceride,total cholesterol and low density lipoprotein cholesterol(LDL)level of the overweight group were higher,while high density lipoprotein cholesterol(HDL)and fasting blood sugar level were lower with no statistical difference(P>0.05).The fasting insulin level and HOMA-IR of overweight group were significantly higher than those of normal weight group(P<0.01).At the phylum level,Firmicutes and Bacteroidetes were the most abundant flora in perimenopausal women's gut microbiota community.There was no significant difference on gut microbial diversity between with normal weight and overweight women(P>0.05).At the class level,it was found that the number of mycetozoan was significantly higher in overweight group than that in normal weight group(P<0.01).At the family and order level,it was found that the number of desulfovibrio was significantly higher in overweight group than that in normal weight group(P<0.01).Conclusion Perimenopausal women gain weight with the production of insulin resistance,which may have a certain relationship with the changes in the structure of the gut microbiota.