地塞米松对RSV诱发哮喘大鼠气道重塑形态学及ICAM-1表达的影响

Effects of Dexamethasone on Airway Remodeling Morphology and ICAM-1 Expression in RSV-Induced Asthmatic Rats

目的:探究地塞米松对呼吸道合胞病毒(respiratory syncytial virus,RSV)诱发哮喘大鼠气道重塑形态学以及细胞间黏附分子-1(intercellular adhesion molecule-1,ICAM-1)表达的影响,论述地塞米松用于防治哮喘的潜在机制。方法:将60只SD雄性大鼠随机分为6组,每组10只,分别为正常组、模型组、泼尼松与沙丁胺醇治疗组、地塞米松高、中、低剂量组。在通过卵清蛋白以及RSV成功构建哮喘大鼠模型后,处死大鼠获取肺组织进行苏木素-伊红(HE)染色,观察各组炎症反应,通过图像分析软件检测各组大鼠气道壁总面积(total bronchial wall area,WAt)、支气管底膜周径(perimeter of the basement membrane,Pbm)以及管壁平滑肌面积(airway bronchial smooth muscle area,WAm)。采用免疫组化法检测各组大鼠肺组织中ICAM-1的表达水平。结果:相较于模型组,泼尼松及沙丁胺醇治疗组、地塞米松中、高剂量组大鼠炎症反应减轻,WAt/Pbm、WAm/Pbm以及ICAM-1表达量相较于模型组也显著降低,差异有统计学意义。结论:地塞米松能够显著缓解哮喘造成的气道重塑症状,可能与其降低肺组织中ICAM-1表达相关。

Objective:To explore the effects of dexamethasone on airway remodeling morphology and intercellular adhesion molecule-1(ICAM-1)expression in respiratory syncytial virus(RSV)induced asthmatic rats,and discuss the potential mechanism of applying dexamethasone in the prevention and treatment of asthma.Methods:60 SD male rats were randomly divided into 6 groups with 10 rats in each group:normal group,model group,prednisone and salbutamol treatment group,dexamethasone high-dose,medium-dose and lowdose groups.After successfully constructing an asthma rat model with ovalbumin and RSV,rats were sacrificed to obtain lung tissue for hematoxylin-eosin(HE)staining to observe the inflammation in each group.Total airway wall area(WAt),perimeter of the basement membrane(Pbm)and airway bronchial smooth muscle area(WAm)of each group were detected by image analysis software.Immunohistochemistry was used to detect the expression level of ICAM-1 in lung tissues of rats in each group.Results:Compared with the model group,the inflammatory response was reduced in prednisone and salbutamol treatment group,dexamethasone high-dose and medium-dose group,and Wat/Pbm,Wam/Pbm and the expression level of ICAM-1 were also significantly decreased compared with the model group,with statistical significance.Conclusion:Dexamethasone can significantly relieve airway remodeling symptoms caused by asthma,which may be related to the decrease of ICAM-1 expression in lung tissue.