血清整合素β3、LIF水平对多囊卵巢综合征病情及合并不孕的评估价值

Evaluation value of serum integrinβ3 and LIF levels in polycystic ovary syndrome and infertility

目的研究血清整合素β3和白血病抑制因子(LIF)水平对多囊卵巢综合征(PCOS)病情及合并不孕的评估价值。方法选择2018年1月至2021年2月重庆市云阳县人民医院收治的PCOS伴不孕患者作为PCOS组(n=120)、排卵正常的非PCOS不孕女性作为对照组(n=100),采用酶联免疫吸附法检测血清整合素β3、LIF水平,采用实验室检测指标黄体生成素(LH)、卵泡刺激素(FSH)、雌二醇(E2)、睾酮(T)、空腹胰岛素(F⁃Ins)、胰岛素抵抗指数(HOMA⁃IR)评估病情,采用超声指标A型内膜比例、子宫内膜厚度评估子宫内膜容受性。结果PCOS组患者血清中整合素β3、LIF的水平均明显低于对照组,差异有统计学意义(P<0.05);血清整合素β3、LIF水平对PCOS伴不孕具有诊断价值,诊断的截断值分别为76.72 ng/mL、8.80 ng/mL(P<0.05);PCOS组中整合素β3≥76.72 ng/mL、LIF≥8.80 ng/mL患者的F⁃Ins、HOMA⁃IR水平均低于整合素β3<76.72 ng/mL、LIF<8.80 ng/mL患者,A型内膜比例、子宫内膜厚度高于整合素β3<76.72 ng/mL、LIF<8.80 ng/mL患者,差异有统计学意义(P<0.05),LH、FSH、E2、T水平与整合素β3<76.72 ng/mL、LIF<8.80 ng/mL患者比较差异无统计学意义(P>0.05)。结论血清整合素β3、LIF水平对PCOS伴不孕具有诊断价值且对胰岛素抵抗、子宫内膜容受性异常具有评估价值。

Objective To study the evaluation value of serum integrin β3 and leukemia inhibitory factor(LIF) levels in polycystic ovary syndrome(PCOS) and infertility. Methods PCOS patients with infertility treated in Yunyang Conunty People ’ s Hospital,Chongqing from January 2018 to February 2021were selected as the PCOS group(n=120),and non PCOS infertile women with normal ovulation were selected as the control group(n=100). The levels of serum integrin β3 and LIF were detected by Elisa. The disease condition of POCS was evaluated by laboratory indicators including luteinizing hormone(LH),follicle stimulating hormone(FSH),estradiol(E2), testosterone(T),fasting insulin(F-Ins)and insulin resistance index(HOMA-IR). The endometrial receptivity was evaluated by ultrasound indexes including type A endometrial ratio and endometrial thickness. Results The levels of integrin β3 and LIF in serum of the PCOS group were significantly lower than those in the control group(P<0.05). The levels of integrin β3 and LIF had diagnostic value for PCOS with infertility,the cutoff values of diagnosis were 76.72 ng/m L and 8.80ng/m L respectively. The levels of F-Ins and HOMA-IR in patients with integrin β3≥76.72 ng/m L and LIF ≥8.80 ng/m L were lower than those of integrin β3<76.72 ng/m L and LIF <8.80 ng/m L,the proportion of type A endometrium and endometrial thickness were higher than those of integrin β3<76.72 ng/m L and LIF<8.80 ng/m L(P<0.05). There was no significant difference in the levels of LH,FSH,E2,T between PCOS patients with different integrins β3 and LIF level(P>0.05). Conclusion Serum integrin β3 and LIF levels have diagnostic value for PCOS with infertility and have evaluation value for insulin resistance and abnormal endometrial receptivity.