膀胱癌中Yes相关蛋白表达与Wnt/β-catenin通路的关系及其临床病理意义

The relationship of Yes associated protein expression and Wnt/β-catenin pathway in bladder cancer and its clinicopathological significance

目的研究膀胱癌中Yes相关蛋白(YAP)表达与Wnt/β-catenin通路的关系及其临床病理意义。方法选择2017年1月至2018年12月期间在湖北省武汉市江夏区中医医院接受手术切除的膀胱癌患者(108例)作为研究对象,同时取癌旁距离≥5 cm的正常组织为对照组。检测膀胱癌组织及癌旁组织中YAP、Wnt1、Wnt3a、β-catenin的表达水平,分析YAP表达与膀胱癌病理特征及Wnt1、Wnt3a、β-catenin表达的关系、随访膀胱癌患者的无进展生存情况和总生存情况,分析YAP表达与生存预后的关系。结果膀胱癌组织的YAP高表达率及mRNA表达水平均高于癌旁组织,差异有统计学意义(P<0.05);膀胱癌YAP高表达患者病理分级Ⅲ级、T3-T4期、淋巴结转移的比例高于YAP低表达患者,膀胱癌组织中Wnt1、Wnt3a、β-catenin的mRNA表达水平高于YAP低表达患者,随访过程中的累积无进展生存率及累积总生存率均低于膀胱癌YAP低表达患者,差异有统计学意义(P<0.05)。结论膀胱癌中YAP表达水平增加与Wnt/β-catenin通路激活及病理进展、生存时间缩短有关。

Objective To study the relationship of Yes associated protein(YAP)expression and Wnt/β-catenin pathway in bladder cancer and its clinicopathological significance.Methods The patients with bladder cancer who underwent surgical resection in Jiangxia District Hospital of Traditional Chinese Medicine from January 2017 to December 2018 were selected as the research objects(n=108).The expression of YAP,Wnt1,Wnt3a,β-catenin in bladder cancer tissues and adjacent tissues were detected.The relationship between YAP and pathological features,Wnt1,Wnt3a,β-catenin expression was analyzed.The progression free survival and overall survival of bladder cancer were followed-up and the relationship between YAP expression and survival prognosis was analyzed.Results The high expression rate and mRNA expression level of YAP in bladder cancer tissues were higher than those in adjacent tissues(P<0.05).In patients with high YAP expression in bladder cancer,the proportion of pathological gradingⅢ,T3-T4 stage and lymph node metastasis were higher than those in patients with low YAP expression,mRNA expression of Wnt1,Wnt3a,β-catenin were higher than those of patients with low YAP expression,the cumulative progression free survival and cumulative overall survival rate during follow-up were lower than those in patients with low YAP expression(P<0.05).Conclusion Increased expression of YAP in bladder cancer is associated with activation of Wnt/β-catenin pathway,pathological progression,and shortened survival time.