摘要
为科学配伍肠道健康食品中的益生菌与益生元,在相同的体外培养条件下评价乳双歧杆菌BL-99、婴儿双歧杆菌YLGB-1496、副干酪乳杆菌K56、副干酪乳杆菌ET-22等4株益生菌对12种益生元的利用特点,高通量筛选典型的合生元组合并分析其发酵特性。研究通过微生物菌落自动化工作站测定所有组合的生长速率,初步筛选特征组合,并与市售典型益生菌乳双歧杆菌BB-12和鼠李糖乳杆菌LGG对照,评价特征组合中益生元对益生菌的促增殖及促产酸能力,并通过益生元降解率和代谢产物分析评价其体外发酵特性。以代谢产物作为主要指标,结果显示不同益生元对益生菌的作用效果差异显著,乳双歧杆菌BL-99与乳糖组合的益生效果较好,而婴儿双歧杆菌YLGB-1496、副干酪乳杆菌K56、副干酪乳杆菌ET-22则与低聚半乳糖组合的益生效果较好。该研究通过高通量组合筛选,为益生菌与益生元的组合提供数据支持,为肠道健康食品开发奠定基础。
To scientifically explore probiotic and prebiotic combinations good for intestinal health,the microbial colony automation workstation was used for high-throughput sequencing.The growth rate from four probiotics(Bifidobacterium lactis BL-99,Bifidobacterium infantis YLGB-1496,Lactobacillus paracasei K56 and Lactobacillus paracasei ET-22)and 12 prebiotics were determined.Commercial probiotics,Bifidobacterium lactis BB-12 and Lactobacillus rhamnosus LGG,were used as control.The results showed that B.lactis BL-99 combined with lactose and B.infantis YLGB-1496,L.paracasei K56,L.paracasei ET-22 combined with galactooligosaccharides showed better effect.This research provides data support for the combination of probiotics and prebiotics through high-throughput sequencing,and lays a foundation for intestinal health food development.
作者
李雅丽
王默涵
赵雯
段素芳
刘伟贤
陈萌
刘义凤
段盛林
洪维鍊
LI Yali;WANG Mohan;ZHAO Wen;DUAN Sufang;LIU Weixian;CHEN Meng;LIU Yifeng;DUAN Shenglin;HONG Weilian(China National Research Institute of Food and Fermentation Industries Co.Ltd.,Beijing 100015,China;Beijing Key Laboratory of the Innovative Development of Functional Staple and the Nutritional Intervention for Chronic Disease,Beijing 100015,China;Yili Innovation Center,Inner Mongolia Yili Industrial Group Co.Ltd.,Hohhot 010110,China;Inner Mongolia Dairy Technology Research Institute Co.Ltd.,Hohhot 010110,China)
出处
《食品与发酵工业》
CAS
CSCD
北大核心
2022年第7期50-57,共8页
Food and Fermentation Industries
关键词
益生菌
益生元
合生元
体外筛选
发酵特性
probiotics
prebiotics
synbiotics
selected in vitro
fermentation properties
