全局转录机器工程调控微生物代谢的应用进展

Progress in the microbial metabolism by global transcription machinery engineering regulation

全局转录机器工程(global transcription machinery engineering,gTME)采用微生物作为“细胞工厂”从转录水平扰动基因表达,直接或间接操纵全局转录调控网络,是一种在基因和细胞水平改造微生物、强化目标性能的高效方法。近年来gTME在增强菌株耐受性、提高应答反应能力、高产代谢产物等方面被广泛应用,较传统方法具有快速优化代谢途径、显著提高产物效率等优势。该文综述了gTME分子机制、在应变工程和代谢工程中最新进展及相配合的进化手段,以及高通量筛选策略,以期为微生物改造和代谢工程研究提供帮助。

Global transcription machinery engineering(gTME)uses microorganisms as“cell factories”to perturb gene expression at the transcription level and directly or indirectly manipulate the global transcriptional regulatory network.It is a powerful technology of engineered microorganisms to enhance target performance at the gene and cell level.In recent years,gTME has been widely used in enhancing strain tolerance,improving response capacity,and increasing the yield of metabolites.Compared with traditional methods,gTME has the advantages of optimizing metabolic pathways rapidly and improving product efficiency significantly.This article reviews the molecular mechanism of gTME,the latest progress in strain engineering and metabolic engineering,and the corresponding evolutionary means and high-throughput screening strategies,with a view to providing the help for microbial modification and metabolic engineering research.