牛IRF7蛋白对牛病毒性腹泻病毒增殖影响的研究

Study on the effect of bovine IRF7 protein on the proliferation of Bovine viral diarrhea virus

为了探索牛干扰素转录调节因子7(IRF7)基因对牛病毒性腹泻病毒(Bovine viral diarrhea virus, BVDV)增殖的影响,试验通过慢病毒包装和RNA干扰等试验构建IRF7基因的干扰和过表达细胞模型,然后运用实时荧光定量PCR扩增和Western-blot验证过表达及干扰效果,最后用BVDV进行感染,通过E2蛋白的表达水平评估IRF7基因与BVDV增殖的关系。结果表明:BVDV感染IRF7基因过表达细胞模型12 h后,E2基因相对表达量显著降低(P<0.05),且随着时间的延长,E2蛋白表达水平逐渐降低;而BVDV感染IRF7基因干扰细胞模型8 h后,E2基因相对表达量显著升高(P<0.05),E2蛋白的表达水平在8 h后逐渐升高,即过表达IRF7基因能够使BVDV增殖水平降低,而干扰IRF7基因的表达则能够使BVDV的增殖水平增加。说明牛IRF7蛋白对牛BVDV的增殖具有抑制作用。

In order to explore the effect of bovine interferon transcription regulator 7(IRF7) gene on the proliferation of Bovine viral diarrhea virus(BVDV), in the experiment, the interference and overexpression cell model of IRF7 gene was constructed through experiments such as lentivirus packaging and RNA interference;then real-time fluorescence quantitative PCR amplification and Western-blot were used to verify the overexpression and interference effect;finally, BVDV was used for infection, and the relationship between IRF7 gene and BVDV reproduction was evaluated by the expression level of E2 protein. The results showed that 12 h after BVDV infected the IRF7 gene overexpression cell model for, the relative expression of E2 gene was significantly reduced(P<0.05), and as time passed, the expression of E2 protein gradually decreased. While, 8 h BVDV infected the IRF7 gene interference model for 8 h, the relative expression of E2 gene increased significantly(P<0.05). The expression level of E2 protein gradually increased, which meant that IRF7 gene could reduce the reproduction level of BVDV, and the interference with the expression of IRF7 gene could increase the reproduction of over-expression of BVDV. The results suggested that IRF7 protein could inhibit the proliferation of BVDV.