飞行时间质谱-GC-MS/MS法测定桂花中β-紫罗兰酮

Determination ofβ-Ionone in Osmanthus fragrans(Thunb.)Lour.by GC-Q-TOF MS and GC-MS/MS

采用水蒸气蒸馏萃取法处理新鲜采摘的桂花,利用飞行时间质谱(GC-Q-TOF/MS)法定性分析β-紫罗兰酮,考察β-紫罗兰酮的碎裂方式,再用气相色谱-三重四级杆串联质谱(GC-MS/MS)多重反应监测(MRM)定量分析桂花中β-紫罗兰酮。该方法在2.00μg/L~100μg/L范围内线性良好,方法检出限为8.67 ng/L。用该方法连续2 d测定新鲜桂花,β-紫罗兰酮均值为5.55 mg/kg。

β-ionone was extracted from fresh Osmanthus fragrans(Thunb.)Lour.by steam distillation,and was qualitatively analyzed by time of fight mass spectrometry(GC-Q-TOF/MS),quantitatively analyzed by gas chromatography-triple quadrupole tandem mass spectrometry(GC-MS/MS)through investigating its fragmentation manner.By optimizing the test conditions,the method had good linearity at 2.00μg/L~100μg/L of the target substance,the method detection limit was 8.67 ng/L.The method was applied in determining fresh Osmanthus fragrans(Thunb.)Lour.for 2 days,β-ionone was detected in two batches of samples,and the average test results was 5.55 mg/kg.