青藏高原岩羊朊蛋白基因及氨基酸序列分析

Sequences of prion protein gene and amino acid of blue sheep on Qinghai-Tibet Plateau

目的 分析岩羊的朊蛋白基因(PRNP)序列,评估岩羊对羊瘙痒病的易感性。方法 参照GenBank上已经发表的绵羊PRNP序列设计引物,对岩羊的PRNP序列进行扩增,基因测序后与GenBank上的11种哺乳动物的PRNP序列比对和分析。结果 测序结果显示岩羊PRNP由771个核苷酸构成,编码256个氨基酸。序列分析结果显示4只岩羊朊蛋白(PrP)氨基酸序列完全同源,并且与野生型绵羊PrP氨基酸序列完全一致。此外,岩羊PrP氨基酸序列与山羊(99.2%)、驯鹿(98.0%)、牛(97.7%)也具有较高的同源性。结论 本次测定的4只岩羊可能和野生型绵羊一样对羊瘙痒病中度易感,岩羊和绵羊种群之间存在相互传播羊瘙痒病的可能。

Objective To analyze the prion protein gene(PRNP) sequence of blue sheep and evaluate the susceptibility of blue sheep to scrapie. Methods The primers were designed according to the PRNP sequence of sheep published on GenBank,and then the PRNP sequence of blue sheep was amplified, sequenced, and compared with the PRNP sequence of 11 species of mammals published on GenBank. Results Gene sequencing results showed that the PRNP of blue sheep consists of 771 nucleotides and encodes 256 amino acids. Sequence analysis showed that the amino acid sequences of prion protein(PrP) in four blue sheep were completely homologous, and were consistent with those of wild-type sheep. In addition, the amino acid sequence of Pr P in blue sheep showed high homology with goat(99.2%), reindeer(98.0%) and cattle(97.7%). Conclusion Four blue sheep enrolled in this study might be susceptible to scrapie as wild-type sheep, and have the potential to transmit scrapie between blue sheep and sheep.