温度对甘青铁线莲种子萌发及相关酶活性的影响

Effects of Temperature on Seed Germination and Related Enzyme Activities of Clematis tangutica(Maxim.)Korsh.

为探究甘青铁线莲[Clematis tangutica(Maxim.)Korsh.]种子萌发的适宜温度和温度对其淀粉酶活性、过氧化物酶活性的影响机制。本文以甘青铁线莲种子为材料,测定种子萌发过程中不同温度下的萌发情况、淀粉酶活性和过氧化物酶活性。结果表明:甘青铁线莲种子对低温有一定的适应性,但最适的萌发温度区间为20~25℃,温度过高或过低都会推迟萌发时间且降低种子的发芽势。低温对淀粉酶活性和过氧化物酶活性影响不大,达到峰值只是时间的差异。高温下淀粉酶活性持续上升,过氧化物酶活性虽然呈缓慢上升趋势但活性值明显偏低,抑制物质积累导致发芽率不高。培养温度为25℃时,甘青铁线莲种子萌发率最高,为76.6%。

In order to explore the effect of suitable temperature and temperature on amylase activity and peroxidase activity of Clematis tangutica(Maxim.)Korsh.The germination,amylase activity and peroxidase activity of Clematis kansuensis seeds at different temperatures were determined.The result showed that:Clematis kansuensis seeds had a certain adaptability to low temperature,but the optimal germination temperature was 20-25℃.The germination time was delayed and the germination potential was decreased when the temperature was too high or too low.Low temperature had little effect on the activity of amylase and peroxidase,and the time to reach the peak value was only the difference of time.Under high temperature,the activity of amylase continued to rise.Although the activity of peroxidase increased slowly,the activity value was obviously low,and the germination rate was not high due to the accumulation of inhibitory substances.The highest germination rate was observed at 25℃,which was 76.6%.