有益菌根真菌及其互作对带叶兜兰试管苗生理生长的影响

Effects of Mycorrhizal Fungi Interaction on the Growth and Physiology of Paphiopedilum hirsutissimum Seedlings in Vitro

为探讨菌根真菌的互作效应,筛选协同互作模式组合,对从野生兜兰新鲜营养根中分离、筛选出的3种菌根真菌(PF02、PF06和PF07),采用单菌接种、双菌混合接种和三菌混合菌种等7种接种方式,开展菌−苗共生培养,筛选出优势菌株PF02和PF07,揭示在实验室环境下接菌方式与接种效应之间的相互联系,分析菌根真菌互作对带叶兜兰植株生长和生理的影响。结果表明:显示菌根真菌互作对宿主植株的生物量、保护酶活性及叶绿素总量具有较好的正向效应。3种双菌混合接种方式PF02−PF06、PF02−PF07及PF06−PF07均能有效促进带叶兜兰生物量增长,尤其是PF02−PF06接种方式,抗氧化酶POD、超氧化物歧化酶SOD、过氧化氢酶CAT活性及叶绿素总量提高显著。选择优化的接种方式,以及能够协同促进、达到共生平衡的最佳接种方式组合,最大限度发挥菌根对宿主植物的益生作用。

To found the interaction effects of orchid mycorrhiza on the growth of orchid seedlings,and screen the combination of synergistic interaction modes,3 mycorrhizal fungi(PF02,PF06 and PF07)isolated from fresh nutritious roots of Paphiopedilum hirsutissimum were studied.Seven inoculations including single and mixed in-oculation of 2 and 3 fungi were adopted,the dominant strains PF02 and PF07 were screened out by fungus-seed-ling symbiotic culture.The dominant strains PF02 and PF07 were screened out by fungus-seedling symbiotic cul-ture.The relationship between inoculation method and inoculation effect in vitro was revealed,and the effects of mycorrhizal fungi interaction on the growth and physiology of P.hirsutissimum were analyzed.The result proves that mycorrhizal fungi interaction was good for biomass accumulation,protective enzyme activity and total chlorophyll content of P.hirsutissimum seedlings,such as inoculation modes with 2 mycorrhizal fungi PF02−PF06,PF02−PF07 and PF06−PF07,especially PF02−PF06,which were effective for the activities of POD,SOD,CAT and total chlorophyll increased significantly.Selecting the optimal inoculation method and the best combination of inoculation methods that could be great significance to P.hirsutissimum production.