7−羟基香豆素的抑菌活性及其稳定性研究

Study on the Antifungal Activity and Stability of 7−hydroxycoumarin

以5种植物病原菌为研究对象,用生长速率法测定7−羟基香豆素对各病菌的抑菌活性;以尖孢镰刀菌为指示菌,测定了7−羟基香豆素抑菌活性在不同条件下的稳定性。结果表明:7−羟基香豆素对5种病原菌均显示出不同程度的抑制作用,尤其是对尖孢镰刀菌作用最为显著,当浓度为1.6 mg/mL时,对尖孢镰刀菌的抑制率可达93.66%。7−羟基香豆素在经过光照、氧化剂和还原剂的处理后,抑菌活性稳定;在酸性条件下,抑菌效果显著增强,而在碱性条件下,抑菌活性下降;在经过Ca^(2+)处理后,7−羟基香豆素的抑菌活性稳定;经过Co^(2+)、Ni^(2+)处理后,抑制效果达到100%,经过Mn^(2+)处理后,抑菌效果显著加强;经过K^(+)处理后,抑菌活性受到抑制。

The growth rate method was used to determine the antifungal activity of 7−hydroxycoumarin against 5 kinds of plant pathogens.Fusarium oxysporum was used as an indicator to determine the stability of the antifungal activity of 7−hydroxycoumarin under different conditions.The results showed that 7−hydroxycoumarin showed different degrees of inhibitory effect on 5 pathogens,especially on F.oxysporum.When the concentration was 1.6 mg/mL,the inhibition rate of F.oxysporum was 93.66%.The antifungal activity of 7−hydroxycoumarin was stable after treatment with light,oxidant and reducing agent,and significantly enhanced under acidic condition,but decreased under alkaline condition;after Ca^(2+)treatment,the antifungal activity of 7−hydroxycoumarin was stable;after Co^(2+)and Ni^(2+)treatment,the inhibitory effect reached 100%,and the antifungal effect was significantly enhanced after Mn^(2+)treatment;after K^(+) treatment,the antifungal activity was inhibited.