植株样本量对云南松子代苗期遗传力估算的影响

Effects of Plant Sample Size on Estimation of Heritability at Seedling Stage of Pinus yunnanensis

为探讨云南松子代苗期遗传力估算最经济有效的调查样本量,以34个云南松1年生半同胞家系苗高、地径为指标,随机抽取部分数据,利用HalfsibSS 1.0软件估算各性状在不同样本量下的遗传力,分析样本量对性状遗传力估算的影响,确定各性状遗传力估算所需的最少样本量。结果表明:对于这34个云南松半同胞家系,不同样本量对苗高和地径的遗传力估算值均有显著影响;当每家系样本数量小于60株时,苗高和地径遗传力估算值波动较大,标准误较大,样本量大于60时,随着样本量的增加,其精确度逐渐增加。因此,对本研究的测定群体而言,要获得精确度较高的遗传力估算值,测定的云南松单株数应每家系不小于60株。

For the purpose of investigating the most economical and effective sample size for estimating heritability of Pinus yunnanensis in seedling stage,the seedling height and ground diameter of seedlings from 34 half-sib families of P.yunnanensis were randomly selected,and some data were estimated by HalfsibSS 1.0 software to estimate the heritability of various characters under different sample sizes,the analysis of the effect of sample size for trait heritability estimates,determine the minimum sample size required by each trait heritability estimates.The results showed that for the 34 P.yunnanensis half-sib families,different sample sizes had significant effects on the heritability estimates of seedling height and ground diameter.When the sample size of each family was less than 60 individuals,the estimated heritability of seedling height and ground diameter fluctuated greatly,and the standard error was large.When the sample size was more than 60,the accuracy of the heritability in-creased gradually with the increase of the sample size.Therefore,in this study,more than 60 individuals should be used for the reliable heritability estimation.