依巴斯汀对Balb/c小鼠特应性皮炎模型的作用研究

Effect of ebastine on Balb/c mouse model of atopic dermatitis

目的:评价抗组胺药依巴斯汀对2,4-二硝基氟苯(DNFB)诱导的特应性皮炎(AD)小鼠模型的疗效及可能机制。方法:将18只6~8周龄Balb/c雌性小鼠随机分为治疗组、阴性对照组、空白对照组,每组6只。DNFB外涂小鼠背部皮肤制备AD小鼠模型,造模2周后治疗组和阴性对照组每天分别予0.2 mL(浓度为0.41 mg/mL)依巴斯汀和等量生理盐水灌胃,每天一次,给药2周后观察小鼠体重及皮损变化、搔抓行为,HE染色观察小鼠皮损表皮厚度及嗜酸粒细胞数目,ELISA法检测小鼠血清白细胞介素4(IL-4)水平。结果:造模小鼠的背部皮肤均出现红斑、水肿、渗液、结痂及皮肤增厚等典型AD皮损表现。治疗组及空白组小鼠体重在实验后增加(P<0.05),阴性对照组小鼠体重无明显变化(P>0.05)。与阴性对照组相比,治疗组在治疗前皮损评分无明显差异(P>0.05),治疗后治疗组皮损评分明显降低(P<0.05),搔抓次数、表皮厚度、嗜酸性粒细胞数目及血清中IL-4水平均降低(P<0.05)。结论:抗组胺药依巴斯汀对AD小鼠模型有一定的治疗作用,可能是通过抑制体内Th2型免疫反应。

Objective: To access the effect and possible mechanism of antihistamine ebastine on atopic dermatitis(AD) mouse model induced by 2,4-dinitrofluorobenzene(DNFB). Methods: Eighteen Balb/c female mice aged 6-8 weeks were randomly divided into treatment group, negative control group and blank group. The AD mouse model was prepared by coating DNFB on the back skin of mice, and the mice in the blank group were not treated. After 2 weeks of modeling, the treatment group was given ebastine of concentration of 0.41 mg/mL, 0.2 mL, by gavage, once a day, and the negative control group was given the same amount of normal saline. After 2 weeks of administration, the changes of body weight, skin lesion and scratching behavior of mice were assessed, the thickness of skin lesions and the number of eosinophils were observed by HE staining, and the level of serum interleukin-4(IL-4) was detected by ELISA. Results: Typical AD lesions such as erythema, edema, exudation, crust and skin thickening appeared in the back skin of the model mice. The weight of mice in the treatment group and the blank group increased after the experiment(P<0.05), but there was no significant change in the negative control group(P>0.05). Compared with the negative control group, there was no significant difference in the score of skin lesion in the treatment group before treatment(P>0.05). After treatment, the score of skin lesion in the treatment group decreased significantly(P<0.05). In addition, the scratching times, epidermal thickness, eosinophil number and serum IL-4 in the treatment group were lower than those in the negative control group(P<0.05). Conclusion: Ebastine has certain therapeutic effect on AD mouse model, which may through inhibiting Th2 immune response in vivo.