益气化浊胶囊预防高脂饮食大鼠胰岛素抵抗的作用初探

A preliminary study on the application of the Yiqi Huazhuo capsules in preventing insulin resistance in rats with high-fat diet

目的:观察益气化浊胶囊对高脂饮食大鼠胰岛素抵抗(Insulin Resistance,IR)的预防作用,并初步探讨其可能的作用机制。方法:将SD大鼠随机分为空白对照组、模型对照组、盐酸二甲双胍组(0.2 g·kg^(-1)·d^(-1))和益气化浊胶囊高、中、低剂量组(2 g·kg^(-1)·d^(-1)、1 g·kg^(-1)·d^(-1)、0.5 g·kg^(-1)·d^(-1)),每组10只。灌胃给药,每日1次,连续8周。给药的同时造模,造模采用高脂饮食喂养诱导IR法。实验期间观察大鼠一般情况,灌胃第8周末,测量体质量、身长,计算Lee’s指数,快速血糖仪测空腹血糖,酶联免疫吸附法检测血清白脂素、胰岛素水平,计算稳态模式评估法胰岛素抵抗指数(Homeostasis Model Assessment of Insulin Resistance Index,HOMA-IR)来评价IR水平。结果:灌胃第8周末时,模型对照组体质量、身长、Lee’s指数、胰岛素水平、HOMA-IR、白脂素水平显著高于空白对照组(P<0.01);而二甲双胍组、益气化浊胶囊高剂量组体质量、身长显著低于模型对照组(P<0.01或P<0.05),二甲双胍组Lee’s指数显著低于模型对照组(P<0.05),二甲双胍组及益气化浊胶囊高、中剂量组胰岛素水平、HOMA-IR、白脂素水平显著低于模型对照组(P<0.01或P<0.05)。结论:益气化浊胶囊可在一定程度上预防高脂饮食大鼠IR,其作用机制可能与血清白脂素水平的降低有关。

Objective:To observe the preventive effect of the Yiqi Huazhuo capsules(益气化浊胶囊)on insulin resistance in rats with high-fat diet,and to explore its possible mechanism.Methods:SD rats were randomly divided into the blank control group,the model control group,the metformin hydrochloride group(0.2 g·kg^(-1)·d^(-1))and the high,middle and low dose groups of the Yiqi Huazhuo capsules(2 g·kg^(-1)·d^(-1),1 g·kg^(-1)·d^(-1),0.5 g·kg^(-1)·d^(-1)),10 rats in each group.Gavage was given once a day,for 8 consecutive weeks.At the same time of administration,the IR rats models were made by high-fat diet feeding induction method.Results:At the end of the 8th week,the body weight,body length,Lee’s index,insulin level,HOMA-IR and asprosin level in the model control group were significantly higher than those in the blank control group(P<0.01),while the body weight,and body length in the metformin hydrochloride group and the high dose group of the Yiqi Huazhuo capsules were significantly lower than the model control group(P<0.01 or P<0.05),the Lee’s index in the metformin hydrochloride group was significantly lower than the model control group(P<0.05),and the insulin level,HOMA-IR,and asprosin level in the metformin hydrochloride group,the high and middle dose groups of the Yiqi Huazhuo capsules were significantly lower than the model control group(P<0.01 or P<0.05).Conclusion:The Yiqi Huazhuo capsules can prevent from IR in rats with high-fat diet to some extent,and its mechanism may be related to the decrease of serum asprosin level.